Open shashank-KU opened 2 years ago
I am getting an error while running PROKKA-
[13:12:05] There are still 2808 unannotated CDS left (started with 2808) [13:12:05] Will use blast to search against /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS with 6 CPUs [13:12:05] Running: cat prokka_GCA_000008285\/PROKKA_04082022\.IS\.tmp\.32109\.faa | parallel --gnu --plain -j 6 --block 73260 --recstart '>' --pipe blastp -query - -db /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS -evalue 1e-30 -qcov_hsp_perc 90 -num_threads 1 -num_descriptions 1 -num_alignments 1 -seg no > prokka_GCA_000008285\/PROKKA_04082022\.IS\.tmp\.32109\.blast 2> /dev/null [13:12:27] Could not run command: cat prokka_GCA_000008285\/PROKKA_04082022\.IS\.tmp\.32109\.faa | parallel --gnu --plain -j 6 --block 73260 --recstart '>' --pipe blastp -query - -db /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS -evalue 1e-30 -qcov_hsp_perc 90 -num_threads 1 -num_descriptions 1 -num_alignments 1 -seg no > prokka_GCA_000008285\/PROKKA_04082022\.IS\.tmp\.32109\.blast 2> /dev/nul
here are the details-
prokka --version prokka 1.14.6 which -a prokka ~/miniconda3/envs/metawrap-env/bin/prokka parallel --version GNU parallel 20210822
prokka --setupdb perl: warning: Setting locale failed. perl: warning: Please check that your locale settings: LANGUAGE = (unset), LC_ALL = (unset), LC_CTYPE = "UTF-8", LANG = "en_US.UTF-8" are supported and installed on your system. perl: warning: Falling back to a fallback locale ("en_US.UTF-8"). [13:20:23] Appending to PATH: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/bin [13:20:23] Cleaning databases in /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.pdb [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.phd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.phi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.phr [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.pin [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.pog [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.pot [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.psd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.psi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.psq [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.ptf [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot.pto [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.pdb [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.phd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.phi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.phr [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.pin [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.pog [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.pot [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.psd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.psi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.psq [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.ptf [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR.pto [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.pdb [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.phd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.phi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.phr [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.pin [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.pog [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.pot [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.psd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.psi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.psq [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.ptf [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS.pto [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.pdb [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.phd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.phi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.phr [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.pin [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.pog [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.pot [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.psd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.psi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.psq [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.ptf [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot.pto [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.pdb [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.phd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.phi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.phr [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.pin [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.pog [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.pot [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.psd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.psi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.psq [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.ptf [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot.pto [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.pdb [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.phd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.phi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.phr [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.pin [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.pog [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.pot [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.psd [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.psi [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.psq [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.ptf [13:20:23] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot.pto [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.pdb [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.phd [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.phi [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.phr [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.pin [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.pog [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.pot [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.psd [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.psi [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.psq [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.ptf [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus.pto [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.pdb [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.phd [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.phi [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.phr [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.pin [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.pog [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.pot [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.psd [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.psi [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.psq [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.ptf [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia.pto [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.pdb [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.phd [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.phi [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.phr [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.pin [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.pog [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.pot [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.psd [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.psi [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.psq [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.ptf [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus.pto [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm.h3f [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm.h3i [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm.h3m [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm.h3p [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea.i1f [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea.i1i [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea.i1m [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea.i1p [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria.i1f [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria.i1i [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria.i1m [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria.i1p [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses.i1f [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses.i1i [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses.i1m [13:20:24] Deleting unwanted file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses.i1p [13:20:24] Cleaning complete. [13:20:24] Looking for 'makeblastdb' - found /mnt/users/shgu/miniconda3/envs/metawrap-env/bin/makeblastdb [13:20:25] Determined makeblastdb version is 002009 from 'makeblastdb: 2.9.0+' [13:20:25] Making kingdom BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Archaea/sprot [13:20:25] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/kingdom\/Archaea\/sprot -logfile /dev/null [13:20:25] Making kingdom BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/sprot [13:20:25] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/kingdom\/Bacteria\/sprot -logfile /dev/null [13:20:27] Making kingdom BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Mitochondria/sprot [13:20:27] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/kingdom\/Mitochondria\/sprot -logfile /dev/null [13:20:28] Making kingdom BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Viruses/sprot [13:20:28] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/kingdom\/Viruses\/sprot -logfile /dev/null [13:20:28] Making kingdom BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/IS [13:20:28] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/kingdom\/Bacteria\/IS -logfile /dev/null [13:20:29] Making kingdom BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/kingdom/Bacteria/AMR [13:20:29] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/kingdom\/Bacteria\/AMR -logfile /dev/null [13:20:29] Making genus BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Enterococcus [13:20:29] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/genus\/Enterococcus -logfile /dev/null [13:20:30] Making genus BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Escherichia [13:20:30] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/genus\/Escherichia -logfile /dev/null [13:20:30] Making genus BLASTP database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/genus/Staphylococcus [13:20:30] Running: makeblastdb -hash_index -dbtype prot -in \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/genus\/Staphylococcus -logfile /dev/null [13:20:31] Looking for 'hmmpress' - found /mnt/users/shgu/miniconda3/envs/metawrap-env/bin/hmmpress [13:20:31] Determined hmmpress version is 003003 from '# HMMER 3.3.2 (Nov 2020); http://hmmer.org/' [13:20:31] Pressing HMM database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm [13:20:31] Running: hmmpress \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/hmm\/HAMAP\.hmm Working... done. Pressed and indexed 1536 HMMs (1536 names). Models pressed into binary file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm.h3m SSI index for binary model file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm.h3i Profiles (MSV part) pressed into: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm.h3f Profiles (remainder) pressed into: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/hmm/HAMAP.hmm.h3p [13:20:33] Looking for 'cmpress' - found /mnt/users/shgu/miniconda3/envs/metawrap-env/bin/cmpress [13:20:33] Determined cmpress version is 001001 from '# INFERNAL 1.1.4 (Dec 2020)' [13:20:33] Pressing CM database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses [13:20:33] Running: cmpress \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/cm\/Viruses Working... done. Pressed and indexed 239 CMs and p7 HMM filters (239 names and 239 accessions). Covariance models and p7 filters pressed into binary file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses.i1m SSI index for binary covariance model file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses.i1i Optimized p7 filter profiles (MSV part) pressed into: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses.i1f Optimized p7 filter profiles (remainder) pressed into: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Viruses.i1p [13:20:34] Pressing CM database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria [13:20:34] Running: cmpress \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/cm\/Bacteria Working... done. Pressed and indexed 1127 CMs and p7 HMM filters (1127 names and 1127 accessions). Covariance models and p7 filters pressed into binary file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria.i1m SSI index for binary covariance model file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria.i1i Optimized p7 filter profiles (MSV part) pressed into: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria.i1f Optimized p7 filter profiles (remainder) pressed into: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Bacteria.i1p [13:20:35] Pressing CM database: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea [13:20:35] Running: cmpress \/net\/fs\-1\/home01\/shgu\/miniconda3\/envs\/metawrap\-env\/db\/cm\/Archaea Working... done. Pressed and indexed 152 CMs and p7 HMM filters (152 names and 152 accessions). Covariance models and p7 filters pressed into binary file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea.i1m SSI index for binary covariance model file: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea.i1i Optimized p7 filter profiles (MSV part) pressed into: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea.i1f Optimized p7 filter profiles (remainder) pressed into: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db/cm/Archaea.i1p [13:20:35] Looking for databases in: /net/fs-1/home01/shgu/miniconda3/envs/metawrap-env/db [13:20:35] * Kingdoms: Archaea Bacteria Mitochondria Viruses [13:20:35] * Genera: Enterococcus Escherichia Staphylococcus [13:20:35] * HMMs: HAMAP [13:20:35] * CMs: Archaea Bacteria Viruses
Any suggestions?
@shashank-KU Did you figure out why? @tseemann prokka was running fine then suddenly I had this error explained here, any advice?
I am getting an error while running PROKKA-
here are the details-
Any suggestions?