Thank you for the development of samclip.
I want to exclude all soft-clipped reads (6 in all) from my dataset.
The line below does not seem to work. No soft-clipped reads are removed.
samtools bamfile.bam | samclip --ref sequence.fa --max 0 --debug | cut -f 6 | sort |uniq -c
Hello Torsten,
Thank you for the development of samclip. I want to exclude all soft-clipped reads (6 in all) from my dataset. The line below does not seem to work. No soft-clipped reads are removed.
samtools bamfile.bam | samclip --ref sequence.fa --max 0 --debug | cut -f 6 | sort |uniq -c
The --invert option, which allows to keep only soft-clipped reads, works fine. Thanks in advance for your feedback, Emilie