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twang15 / Long-read-RNA

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Biology-6: RNA-editing #10

Open twang15 opened 3 years ago

twang15 commented 3 years ago

Enzyme, ADAR (proof-read and correct mistakes in RNA)

ADAR editing enzymes are found in all multicellular animals and are conserved in sequence and protein organization. The number of ADAR genes differs between animals, ranging from three in mammals to one in Drosophila. ADAR is also alternatively spliced to generate isoforms that can differ significantly in enzymatic activity. Therefore, to study the enzyme in vitro, it is essential to have an easy and reliable method of expressing and purifying recombinant ADAR protein. To add to the complexity of RNA editing, the number of transcripts that are edited by ADARs differs in different organisms.

  1. ADAR: an enzyme proofreading and correcting mistakes in RNA

guide RNA, and its role in RNA editing

Long-read RNA sequencing

It is possible to quantify site‐specific RNA editing by sequencing of clones derived from RT‐PCR products.

From Fereshteh:

  1. Linked Reads Genomics - 10X Genomics
  2. Unique Molecule Identifier
  3. UMI-count modeling and differential expression analysis for single-cell RNA sequencing
  4. UMI reveal a novel sequencing artefact with implications for RNA-seq based gene expression analysis

From Hagen

  1. 2015-Nature-Comprehensive transcriptome analysis using synthetic long-read sequencing reveals molecular co-association of distant splicing events SLR-RNASeq

Barcode

Well

single cell

single molecule

twang15 commented 3 years ago

Antibody and Antigen, PCR test

https://www.technologynetworks.com/immunology/articles/antigen-vs-antibody-what-are-the-differences-293550