The focus of our study is using one of the best available long-read RNA-seq data to better understand :
A) tissue and cell type specific RNA editing (coordinated, non-coordinated and allelic expression-related , ect)
B) tissue and cell type specific RNA splicing regulation (coordinated, non-coordinated and allelic expression-related , ect)
1-For Tissue: we can compare bulk long- read RNA-seq from our human adult brain, fetal brain, and the 3 encode cell lines data
2-for cell types, we can use differentiated and undifferentiated cortical neurons from a patient, whose Whole exome seq shows a pathogenic ADAR variant.
As for allelic gene expression:
Please first kindly watch the two videos,
then read the Wikipedia,
then think of a person who has two allele on ADAR gene, one allele is dysfunctional, and one works and then what Haugen has suggested regarding ADAR allelic gene expression and its impact on editing at single cell level.
I also highly suggest reading the attached paper, as it gives a broad review on RNA editing and some of relevant techniques and statistical tools.
I really hope we pass through this last step and can use the established tool to look for tissue ad cell type specific RNA editing in our all our dataset and aim to publish before March 2022.
The focus of our study is using one of the best available long-read RNA-seq data to better understand : A) tissue and cell type specific RNA editing (coordinated, non-coordinated and allelic expression-related , ect)
B) tissue and cell type specific RNA splicing regulation (coordinated, non-coordinated and allelic expression-related , ect)
1-For Tissue: we can compare bulk long- read RNA-seq from our human adult brain, fetal brain, and the 3 encode cell lines data
2-for cell types, we can use differentiated and undifferentiated cortical neurons from a patient, whose Whole exome seq shows a pathogenic ADAR variant.
As for allelic gene expression: Please first kindly watch the two videos, then read the Wikipedia, then think of a person who has two allele on ADAR gene, one allele is dysfunctional, and one works and then what Haugen has suggested regarding ADAR allelic gene expression and its impact on editing at single cell level.
I also highly suggest reading the attached paper, as it gives a broad review on RNA editing and some of relevant techniques and statistical tools.
I really hope we pass through this last step and can use the established tool to look for tissue ad cell type specific RNA editing in our all our dataset and aim to publish before March 2022.
Videos