I have a question about a warning related to bootstrapping that occurs when 'len(Y) < 30'.
In your code, Y is calculated as the number of species IDs in the 'otu_table.csv'.
Therefore, in the provided example file 'cdiff-counts.csv', it is calculated as 'len(Y) = 5'.
Thus, since 'len(Y) < 30' in the example, a warning about the reliability of bootstrapping is being generated.
In similar research, is it necessary to conduct daily fecal surveys of more than 30 individuals for practical application?
I have a question about a warning related to bootstrapping that occurs when 'len(Y) < 30'.
In your code, Y is calculated as the number of species IDs in the 'otu_table.csv'. Therefore, in the provided example file 'cdiff-counts.csv', it is calculated as 'len(Y) = 5'. Thus, since 'len(Y) < 30' in the example, a warning about the reliability of bootstrapping is being generated. In similar research, is it necessary to conduct daily fecal surveys of more than 30 individuals for practical application?