uec / Issue.Tracker

Automatically exported from code.google.com/p/usc-epigenome-center
0 stars 0 forks source link

evaluate novoalign #241

Closed GoogleCodeExporter closed 8 years ago

GoogleCodeExporter commented 8 years ago
download novoalign
install

try on bis data

Original issue reported on code.google.com by zack...@gmail.com on 4 May 2012 at 6:26

GoogleCodeExporter commented 8 years ago
installed novoalign, 

created wrapper

tested on 200K line fastq

modified existing bisulfite worlflow to optionally support novoalign.

usage: 
#bsmap
Sample.7.Workflow = bisulfite

#novoalign
Sample.7.Workflow = bisulfite-novo

currently testing on flowcell C0D, from my shorter test, novoalign is quite 
slow...

Original comment by zack...@gmail.com on 15 May 2012 at 12:13

GoogleCodeExporter commented 8 years ago
yaping was in contact with the guys from novoalign and the following cmd lines 
is being tested:

$novo -d $refNovo -f $read1 $read2 -F ILMFQ -b 2 -o SAM -k -t 240 -l 50  > 
$outfileSAM

Original comment by zack...@gmail.com on 21 May 2012 at 7:06

GoogleCodeExporter commented 8 years ago
lung tumor / lung normal, all lanes are being reprocessed with novoalign. 

Original comment by zack...@gmail.com on 21 May 2012 at 9:28

GoogleCodeExporter commented 8 years ago
I've created a walker to compare the differences between novo and bsmap

running on lung tumor

Original comment by zack...@gmail.com on 5 Jun 2012 at 8:02

GoogleCodeExporter commented 8 years ago
closing this redundant ticket in favor of later, more detailed tickets 
regarding novoalign

Original comment by zack...@gmail.com on 30 Jul 2012 at 8:29