A warning message like this:
WARNING: Could not calculate AD ratio from Record(CHROM=gi|15829254|ref|NC_002695.1|, POS=267176, REF=C, ALT=[G, T]) POS: 267176
Can you provide some insight of this warning message? I am trying to understand this msg's implication, if this will be called as a SNP? I noticed Tim opened an issue last year about mixed positions allowed cutoff, are they related?
The AD ratio cannot be calculated if there are two different ref bases called. If you filter your vcf on the AD ratio, this position will fail that filter, so an N would be put in at that position.
A warning message like this: WARNING: Could not calculate AD ratio from Record(CHROM=gi|15829254|ref|NC_002695.1|, POS=267176, REF=C, ALT=[G, T]) POS: 267176
Can you provide some insight of this warning message? I am trying to understand this msg's implication, if this will be called as a SNP? I noticed Tim opened an issue last year about mixed positions allowed cutoff, are they related?