Open tiagobrc opened 1 year ago
Have you been able to solve this issue? I have the same problem that 3' barcodes are not detected even though I believe that they are correctly specified
Hi, please send me a sample of your fastq and your barcodes csv
Hi thanks for offering this! Hope it is a simple issue with the 3'barcode. Please excuse the labelling of the barcode file, but I think I went through too many versions. As shown in post below. I solved it myself, thanks for offering your help!!
OK, I solved it myself. It is the min_trim=3 default setting. It removed the last three nucleotides of the barcode and therefore it could not be matched. I will remove my data files from above.
I will try this suggestion.
Hi, Thanks for this fantastic tool!
I have been testing this tool on our pipelines and got into trouble when trying to demultiplex the 3' barcode on the second read pair.
The 5' demultiplexing works as a charm. So no problems regarding that.
I have a second-read pair that used a very similar barcode system.
Here is what my barcode.csv looks like.
I have a few questions that could not find in the manual or it wasn't clear enough.
1) Will Ultraplex automatically reverse complement my 3'bc for detection? 2) I actually have 96 barcodes combined with 16 sets ( these let me multiplex 16 different plates). Is there an easier way of creating the barcode.csv file? Instead of adding all combinations separated by a comma?
Read1
Read2