Closed sajvanderzeeuw closed 2 years ago
Hello,
I'd recommend to start start the jar file. Either double click or use java -jar dist/Gepard-140.jar from the command line.
Best, Thomas
ue
Prof. Dr. Thomas Rattei bbc CUBE - Division of Computational Systems Biology cbc Dept. of Microbiology and Ecosystem Science ec cce.cc University of Vienna bbbc ccuc....b Althanstrasse 14 c bb........ce 1090 Vienna, Austria ue........cu Phone +43 1 4277 76680 ce......c bbue Mobile +43 664 60277 76680 e..ccucc be e-mail thomas.rattei@univie.ac.at bcc web cube.univie.ac.at cbb ____ u c
Am 08.02.2016 um 20:22 schrieb sajvanderzeeuw notifications@github.com:
Hi,
First of all thanks of making this nice tool In your HTML tutorial you distribute with Gepard, i see that you also support an commandline version of Gepard which should be started from a sh file from the toplevel of the Gepard dir Can you please tell me where i can find that script Since when i clone directly from here, i get nothing As you can see in the tree below Hope you can help me out Thanks,
Best regards,
Sander
├── dist │ └── Gepard-140jar ├── Gepardjardesc ├── LICENSE ├── LICENSEtxt ├── READMEmd ├── README run without JARtxt ├── READMEtxt ├── resources │ ├── help │ │ ├── helpxml │ │ ├── referencetxt │ │ └── usermsgtxt │ ├── images │ │ ├── exportgif │ │ ├── gepardgif │ │ ├── logogif │ │ ├── zoomfullgif │ │ ├── zoomingif │ │ └── zoomoutgif │ ├── matrices │ │ ├── blosum62mat │ │ ├── ednamat │ │ ├── ednaorigmat │ │ ├── matricesxml │ │ ├── pam250mat │ │ └── protidentitymat │ └── tutorialhtml ├── src │ ├── matrices │ │ ├── blosum62mat │ │ ├── ednamat │ │ ├── ednaorigmat │ │ ├── matricesxml │ │ ├── pam250mat │ │ └── protidentitymat │ └── org │ └── gepard │ ├── client │ │ ├── AutoParametersjava │ │ ├── BrowserLauncherjava │ │ ├── ClientGlobalsjava │ │ ├── cmdline │ │ │ ├── CommandLineArgumentsjava │ │ │ ├── CommandLinejava │ │ │ └── InvalidArgumentsExceptionjava │ │ ├── Configjava │ │ ├── Controllerjava │ │ ├── DotplotInfojava │ │ ├── DrawTargetjava │ │ ├── GeneNamesjava │ │ ├── InvalidParamSetExceptionjava │ │ ├── Plotterjava │ │ ├── Startjava │ │ ├── SubstMatrixFilejava │ │ ├── SubstMatrixListjava │ │ └── userinterface │ │ ├── AboutDialogjava │ │ ├── ContainerWindowjava │ │ ├── ControlPaneljava │ │ ├── CustomComboBoxjava │ │ ├── DrawPaneljava │ │ ├── ExampleFileFilterjava │ │ ├── HelpTextsjava │ │ ├── InfoPaneljava │ │ ├── MainPaneljava │ │ ├── ProxyDialogjava │ │ ├── StatusDialogjava │ │ ├── UserMessageDialogjava │ │ └── WindowPosjava │ └── common │ ├── AbortionCheckerjava │ ├── Base64java │ ├── CompressedDotMatrixjava │ ├── Compressionjava │ ├── DotMatrixCallbackjava │ ├── DotMatrixjava │ ├── FASTAReaderjava │ ├── FASTAWriterjava │ ├── GenSAFilejava │ ├── GenSAFileVmatchjava │ ├── InvalidFASTAFileExceptionjava │ ├── InvalidSubMatFileExceptionjava │ ├── LEDataInputStreamjava │ ├── LEDataOutputStreamjava │ ├── ParameterSetjava │ ├── SAXFinishedExceptionjava │ ├── Sequencejava │ ├── SubstitutionMatrixjava │ ├── SuffixArrayjava │ └── VmatchConverterjava └── tutorialhtml
13 directories, 80 files```
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HI Thomas,
Thanks for the quick reply! I know how to start the jar file but that gives me an GUI, i basically need the functionalities directly on the commandline which enables me to run this tool on multiple comparisons at once. I was hoping that the bash script you are talking about in the README, and tutorial in this repository would give me that functionality.
Best,
Sander
Hi Sander,
sorry, the readme.txt file was not up-to-date in that point. For command line functionality please run gepard this way:
java -cp Gepard-1.40.jar org.gepard.client.cmdline.CommandLine
Best, Thomas
ue
Prof. Dr. Thomas Rattei bbc CUBE - Division of Computational Systems Biology cbc Dept. of Microbiology and Ecosystem Science ec cce.cc University of Vienna bbbc ccuc....b Althanstrasse 14 c bb........ce 1090 Vienna, Austria ue........cu Phone +43 1 4277 76680 ce......c bbue Mobile +43 664 60277 76680 e..ccucc be e-mail thomas.rattei@univie.ac.at bcc web cube.univie.ac.at cbb ____ u c
Am 09.02.2016 um 13:07 schrieb sajvanderzeeuw notifications@github.com:
HI Thomas,
Thanks for the quick reply! I know how to start the jar file but that gives me an GUI, i basically need the functionalities directly on the commandline which enables me to run this tool on multiple comparisons at once. I was hoping that the bash script you are talking about in the README, and tutorial in this repository would give me that functionality.
Best,
Sander
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HI Thomas,
Thanks for answering so fast. Would be a nice idea to update the
tutorial indeed, or just add one small .sh script which runs java -cp Gepard-1.40.jar org.gepard.client.cmdline.CommandLine
.
Best and again thanks!
Sander
On 09-02-16 15:44, rattei wrote:
Hi Sander,
sorry, the readme.txt file was not up-to-date in that point. For command line functionality please run gepard this way:
java -cp Gepard-1.40.jar org.gepard.client.cmdline.CommandLine
Best, Thomas
ue Prof. Dr. Thomas Rattei bbc CUBE - Division of Computational Systems Biology cbc Dept. of Microbiology and Ecosystem Science ec cce.cc University of Vienna bbbc ccuc....b Althanstrasse 14 c bb........ce 1090 Vienna, Austria ue........cu Phone +43 1 4277 76680 ce......c bbue Mobile +43 664 60277 76680 e..ccucc be e-mail thomas.rattei@univie.ac.at bcc web cube.univie.ac.at cbb ____ u c
Am 09.02.2016 um 13:07 schrieb sajvanderzeeuw notifications@github.com:
HI Thomas,
Thanks for the quick reply! I know how to start the jar file but that gives me an GUI, i basically need the functionalities directly on the commandline which enables me to run this tool on multiple comparisons at once. I was hoping that the bash script you are talking about in the README, and tutorial in this repository would give me that functionality.
Best,
Sander
— Reply to this email directly or view it on GitHub.
— Reply to this email directly or view it on GitHub https://github.com/univieCUBE/gepard/issues/1#issuecomment-181897074.
If it helps others... I had similar issues as above. I want to run many plots and would prefer cli. I did not find the right way to give the matrix which is one of the required inputs I found the fix by copying the matrices folder from the src to the gepard folder and provide the full path to the edna.mat as in the example Thanks for the great tool S
Hello Stephane,
you did not provide the -matrix parameter, which is mandatory. If you add it, like in this test, it works:
java -cp /apps/gepard/1.40/dist/Gepard-1.40.jar org.gepard.client.cmdline.CommandLine -seq1 ref1.fasta -seq2 ref2.fasta -matrix /apps/gepard/1.40/resources/matrices/edna.mat -word 100 -window 1000 -outfile gepard_ref1_vs_ref2.png
Best, Thomas
ue
Prof. Dr. Thomas Rattei bbc CUBE - Division of Computational Systems Biology cbc Dept. of Microbiology and Ecosystem Science ec cce.cc University of Vienna bbbc ccuc....b Althanstrasse 14 c bb........ce 1090 Vienna, Austria ue........cu Phone +43 1 4277 76680 ce......c bbue Mobile +43 664 60277 76680 e..ccucc be e-mail thomas.rattei@univie.ac.at bcc web cube.univie.ac.at cbb ____ u c
Am 21.04.2017 um 13:03 schrieb Stephane Plaisance notifications@github.com:
I have similar issues as above. I want to run many plots and would prefer cli. my command below does not complain and no output is created do I need to specify all parameters or are some set by default? the -word and -window were defined from a good GUI run Thanks for your help
java -cp /opt/biotools/gepard/gepard.jar org.gepard.client.cmdline.CommandLine -seq1 "ref1.fasta" -seq2 "ref2.fasta" -word 100 -window 1000 -outfile "gepard_ref1_vs_ref2.png"
Gepard 1.40 final - command line mode
Reference: Krumsiek J, Arnold R, Rattei T Gepard: A rapid and sensitive tool for creating dotplots on genome scale. Bioinformatics 2007; 23(8): 1026-8. PMID: 17309896
Parameters are supplied as -name value
Required parameters: -seq1: first sequence file -seq2: second sequence file -matrix: substitution matrix file -outfile: output file name
Dotplot image parameters: -maxwidth: maximum width of the generated image (default: 750) -maxheight: maximum height of the generated image (default: 750) -zoom: specify a zoom factor for the dotplot note: you can only use maxwidth/maxheight OR zoom when using maxwidth/maxheight the program tries to generate the largest possible dotplot within the given bounds -format: output format, one of: 'png', 'jpg', 'bmp' (default:PNG)
Dotplot computation parameters: -secondcomp use complementary of second sequence -word: word length for suffix array mode (default: 10) -window: window size for ordinary dotplot mode (default: 0) if a window value and no word value is specified, word=0 is assumed
Suffix array parameters: -safile load suffix array from file instead of calculating it -sasecondseq the suffix array is for the second sequence if -sasecondseq is NOT specified, the suffix array will be used for first sequence
Coordinate parameters (absolute values of % values) -from1,-to1 coordinates of first sequence -from2,-to2 coordinates of second sequence if these parameters are not specified the full sequence will be used
Display parameters: -lower lower limit for dot intensity (in %) -upper upper limit for dot intensity (in %) -greyscale greyscale start value (in %)
Miscellaneous: -silent generate no output (except error messages)
all tried commands execute like this even only specifying seq1 and seq2
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Thanks a lot Thomas,
I corrected my comment after finding out I was missing the matrix indeed. I have it now working, the problem was that I downloaded only the jar and did not have the matrices folder with it. I now copied it from the src folder and link to the edna.mat in my cmd great tool!!
Best Stephane
Stéphane Plaisance – Staff Scientist | Bioinformatician VIB Nucleomics Core Herestraat 49 – Post Box 816 – 3000 Leuven – Belgium O&N4 Building – 8th Floor – Room 08.440 Tel. +32 16 37 31 26 Lync. +32 16 32 00 60 www.nucleomics.be http://www.nucleomics.be/ http://www.nucleomics.be/ http://www.nucleomics.be/ http://www.nucleomics.be/ http://www.vib.be/
On 21 Apr 2017, at 13:33, rattei notifications@github.com wrote:
Hello Stephane,
you did not provide the -matrix parameter, which is mandatory. If you add it, like in this test, it works:
java -cp /apps/gepard/1.40/dist/Gepard-1.40.jar org.gepard.client.cmdline.CommandLine -seq1 ref1.fasta -seq2 ref2.fasta -matrix /apps/gepard/1.40/resources/matrices/edna.mat -word 100 -window 1000 -outfile gepard_ref1_vs_ref2.png
Best, Thomas
ue Prof. Dr. Thomas Rattei bbc CUBE - Division of Computational Systems Biology cbc Dept. of Microbiology and Ecosystem Science ec cce.cc University of Vienna bbbc ccuc....b Althanstrasse 14 c bb........ce 1090 Vienna, Austria ue........cu Phone +43 1 4277 76680 ce......c bbue Mobile +43 664 60277 76680 e..ccucc be e-mail thomas.rattei@univie.ac.at bcc web cube.univie.ac.at cbb ____ u c
Am 21.04.2017 um 13:03 schrieb Stephane Plaisance notifications@github.com:
I have similar issues as above. I want to run many plots and would prefer cli. my command below does not complain and no output is created do I need to specify all parameters or are some set by default? the -word and -window were defined from a good GUI run Thanks for your help
java -cp /opt/biotools/gepard/gepard.jar org.gepard.client.cmdline.CommandLine -seq1 "ref1.fasta" -seq2 "ref2.fasta" -word 100 -window 1000 -outfile "gepard_ref1_vs_ref2.png"
Gepard 1.40 final - command line mode
Reference: Krumsiek J, Arnold R, Rattei T Gepard: A rapid and sensitive tool for creating dotplots on genome scale. Bioinformatics 2007; 23(8): 1026-8. PMID: 17309896
Parameters are supplied as -name value
Required parameters: -seq1: first sequence file -seq2: second sequence file -matrix: substitution matrix file -outfile: output file name
Dotplot image parameters: -maxwidth: maximum width of the generated image (default: 750) -maxheight: maximum height of the generated image (default: 750) -zoom: specify a zoom factor for the dotplot note: you can only use maxwidth/maxheight OR zoom when using maxwidth/maxheight the program tries to generate the largest possible dotplot within the given bounds -format: output format, one of: 'png', 'jpg', 'bmp' (default:PNG)
Dotplot computation parameters: -secondcomp use complementary of second sequence -word: word length for suffix array mode (default: 10) -window: window size for ordinary dotplot mode (default: 0) if a window value and no word value is specified, word=0 is assumed
Suffix array parameters: -safile load suffix array from file instead of calculating it -sasecondseq the suffix array is for the second sequence if -sasecondseq is NOT specified, the suffix array will be used for first sequence
Coordinate parameters (absolute values of % values) -from1,-to1 coordinates of first sequence -from2,-to2 coordinates of second sequence if these parameters are not specified the full sequence will be used
Display parameters: -lower lower limit for dot intensity (in %) -upper upper limit for dot intensity (in %) -greyscale greyscale start value (in %)
Miscellaneous: -silent generate no output (except error messages)
all tried commands execute like this even only specifying seq1 and seq2
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Hello Stephane,
these are no special matrices, you might just use them e.g. from a commandline blast installation.
best, Thomas
Am 21.04.2017 um 13:36 schrieb Stephane Plaisance notifications@github.com:
Thanks a lot Thomas,
I corrected my comment after finding out I was missing the matrix indeed. I have it now working, the problem was that I downloaded only the jar and did not have the matrices folder with it. I now copied it from the src folder and link to the edna.mat in my cmd great tool!!
Best Stephane
Stéphane Plaisance – Staff Scientist | Bioinformatician VIB Nucleomics Core Herestraat 49 – Post Box 816 – 3000 Leuven – Belgium O&N4 Building – 8th Floor – Room 08.440 Tel. +32 16 37 31 26 Lync. +32 16 32 00 60 www.nucleomics.be http://www.nucleomics.be/ http://www.nucleomics.be/ http://www.nucleomics.be/ http://www.nucleomics.be/ http://www.vib.be/
On 21 Apr 2017, at 13:33, rattei notifications@github.com wrote:
Hello Stephane,
you did not provide the -matrix parameter, which is mandatory. If you add it, like in this test, it works:
java -cp /apps/gepard/1.40/dist/Gepard-1.40.jar org.gepard.client.cmdline.CommandLine -seq1 ref1.fasta -seq2 ref2.fasta -matrix /apps/gepard/1.40/resources/matrices/edna.mat -word 100 -window 1000 -outfile gepard_ref1_vs_ref2.png
Best, Thomas
ue Prof. Dr. Thomas Rattei bbc CUBE - Division of Computational Systems Biology cbc Dept. of Microbiology and Ecosystem Science ec cce.cc University of Vienna bbbc ccuc....b Althanstrasse 14 c bb........ce 1090 Vienna, Austria ue........cu Phone +43 1 4277 76680 ce......c bbue Mobile +43 664 60277 76680 e..ccucc be e-mail thomas.rattei@univie.ac.at bcc web cube.univie.ac.at cbb ____ u c
Am 21.04.2017 um 13:03 schrieb Stephane Plaisance notifications@github.com:
I have similar issues as above. I want to run many plots and would prefer cli. my command below does not complain and no output is created do I need to specify all parameters or are some set by default? the -word and -window were defined from a good GUI run Thanks for your help
java -cp /opt/biotools/gepard/gepard.jar org.gepard.client.cmdline.CommandLine -seq1 "ref1.fasta" -seq2 "ref2.fasta" -word 100 -window 1000 -outfile "gepard_ref1_vs_ref2.png"
Gepard 1.40 final - command line mode
Reference: Krumsiek J, Arnold R, Rattei T Gepard: A rapid and sensitive tool for creating dotplots on genome scale. Bioinformatics 2007; 23(8): 1026-8. PMID: 17309896
Parameters are supplied as -name value
Required parameters: -seq1: first sequence file -seq2: second sequence file -matrix: substitution matrix file -outfile: output file name
Dotplot image parameters: -maxwidth: maximum width of the generated image (default: 750) -maxheight: maximum height of the generated image (default: 750) -zoom: specify a zoom factor for the dotplot note: you can only use maxwidth/maxheight OR zoom when using maxwidth/maxheight the program tries to generate the largest possible dotplot within the given bounds -format: output format, one of: 'png', 'jpg', 'bmp' (default:PNG)
Dotplot computation parameters: -secondcomp use complementary of second sequence -word: word length for suffix array mode (default: 10) -window: window size for ordinary dotplot mode (default: 0) if a window value and no word value is specified, word=0 is assumed
Suffix array parameters: -safile load suffix array from file instead of calculating it -sasecondseq the suffix array is for the second sequence if -sasecondseq is NOT specified, the suffix array will be used for first sequence
Coordinate parameters (absolute values of % values) -from1,-to1 coordinates of first sequence -from2,-to2 coordinates of second sequence if these parameters are not specified the full sequence will be used
Display parameters: -lower lower limit for dot intensity (in %) -upper upper limit for dot intensity (in %) -greyscale greyscale start value (in %)
Miscellaneous: -silent generate no output (except error messages)
all tried commands execute like this even only specifying seq1 and seq2
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Would it be possible to start gepard from command line with parameters to the 2 fasta files which need comparison? Are there instructions for the command line parameters if there are any?
Hello,
When you run the gepard application from the command line, as explained in the readme.txt, it shows the usage:
java -cp dist/Gepard-1.40.jar org.gepard.client.cmdline.CommandLine
Gepard 1.40 final - command line mode
Reference: Krumsiek J, Arnold R, Rattei T Gepard: A rapid and sensitive tool for creating dotplots on genome scale. Bioinformatics 2007; 23(8): 1026-8. PMID: 17309896
Parameters are supplied as -name value
Required parameters: -seq1: first sequence file -seq2: second sequence file -matrix: substitution matrix file -outfile: output file name
Dotplot image parameters: -maxwidth: maximum width of the generated image (default: 750) -maxheight: maximum height of the generated image (default: 750) -zoom: specify a zoom factor for the dotplot note: you can only use maxwidth/maxheight OR zoom when using maxwidth/maxheight the program tries to generate the largest possible dotplot within the given bounds -format: output format, one of: 'png', 'jpg', 'bmp' (default:PNG)
Dotplot computation parameters: -secondcomp use complementary of second sequence -word: word length for suffix array mode (default: 10) -window: window size for ordinary dotplot mode (default: 0) if a window value and no word value is specified, word=0 is assumed
Suffix array parameters: -safile load suffix array from file instead of calculating it -sasecondseq the suffix array is for the second sequence if -sasecondseq is NOT specified, the suffix array will be used for first sequence
Coordinate parameters (absolute values of % values) -from1,-to1 coordinates of first sequence -from2,-to2 coordinates of second sequence if these parameters are not specified the full sequence will be used
Display parameters: -lower lower limit for dot intensity (in %) -upper upper limit for dot intensity (in %) -greyscale greyscale start value (in %)
Miscellaneous: -silent generate no output (except error messages)
Best regards, Thomas Rattei
Prof. Dr. Thomas Rattei CUBE - Division of Computational Systems Biology Dept. of Microbiology and Ecosystem Science University of Vienna Althanstrasse 14 1090 Vienna, Austria Phone +43 1 4277 91280 Mobile +43 664 60277 91280 e-mail thomas.rattei@univie.ac.atmailto:thomas.rattei@univie.ac.at web cube.univie.ac.at
On 23.01.2020, at 19:11, Dominik Ivosevic notifications@github.com<mailto:notifications@github.com> wrote:
Would it be possible to start gepard from command line with parameters to the 2 fasta files which need comparison? Are there instructions for the command line parameters if there are any?
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Hi,
First of all thanks of making this nice tool. In your .HTML tutorial you distribute with Gepard, i see that you also support an commandline version of Gepard which should be started from a .sh file from the toplevel of the Gepard dir. Can you please tell me where i can find that script. Since when i clone directly from here, i get nothing. As you can see in the tree below. Hope you can help me out. Thanks,
Best regards,
Sander