Open veedeeve opened 2 years ago
Tried to perform a trim on DRR099963. (renamed to fimi)
Input Code:
trimmomatic PE -phred33 fimi_R1.fastq fimi_R2.fastq \ fimi_R1_paired.fastq.gz fimi_R1_unpaired.fastq.gz \ fimi_R2_paired.fastq.gz fimi_R2_unpaired.fastq.gz \ LEADING:10 TRAILING:10 SLIDINGWINDOW:5:20 MINLEN:100
Output:
TrimmomaticPE: Started with arguments: -phred33 fimi_R1.fastq fimi_R2.fastq fimi_R1_paired.fastq.gz >fimi_R1_unpaired.fastq.gz fimi_R2_paired.fastq.gz >fimi_R2_unpaired.fastq.gz LEADING:10 TRAILING:10 >SLIDINGWINDOW:5:20 MINLEN:100 Multiple cores found: Using 4 threads Input Read Pairs: 195 Both Surviving: 0 (0.00%) Forward Only >Surviving: 0 (0.00%) Reverse Only Surviving: 195 (100.00%) >Dropped: 0 (0.00%)
Unsure on why the input read pairs are incorrect. A different person ran the same code, was able to get a complete trim.
Is there anything that I could check? Or is the code incorrect?
Tried to perform a trim on DRR099963. (renamed to fimi)
Input Code:
Output:
Unsure on why the input read pairs are incorrect. A different person ran the same code, was able to get a complete trim.![Picture1](https://user-images.githubusercontent.com/100176672/164307444-6447c97b-ee8b-4d3d-96da-35a83be64a60.png)
Is there anything that I could check? Or is the code incorrect?