I realized that after performing trimmomatic, I have reads in my file R1_p (forward paired), that aren't in my R2_p (reverse paired) file. The number of reads in both files is the same, what could be happening?
In this case, the first read is found in R1_p but not in the R2_p file. Then the next ids are the same...
Hi everyone,
I realized that after performing trimmomatic, I have reads in my file R1_p (forward paired), that aren't in my R2_p (reverse paired) file. The number of reads in both files is the same, what could be happening?
In this case, the first read is found in R1_p but not in the R2_p file. Then the next ids are the same...
head of R1_p file: @M03132:284:000000000-KM9YY:1:1101:14323:2916 1:N:0:TCGACTTG+AGCCTTAA GGTACTGGTTGGACAGTGTATCCTCCTTTATCAGGTATTCAATCACATTCGGGAGGTTCTGTAGATTTAGCTATTTTCAGTTTACATTTAGCAGGAACTTCTTCTTTATTGGGTTCT ATAAATTTTATTACAACTATTTTTAATATGAGAGTTCCTGGAATGGAAATGCATAAAATACCCTTATTTGTATGAGCGATTTTAATAACTGCGTTTTTATTGTTATTATCACTACCT GTTTTAGCTG + AAABBFFFFFBBGGGGGGDGGGHFHHHHHHHHHHHGGHGHHHHHGHFHHHHHGGGGGHHFGGHHGHHHHGHHHHHHHHHBGFFHHFHHHHHHHHFECGHFHFFHBHHHHHHHEGGHG HHHHFHHHHHHHHHHHHHHHHHGHHHHHGHHFFHHHHHHHHFGHBG2FGHBDDGGGHHGFHFHHHBHHHBGDHEB>G?CHHGHHEHHHBGFFDGGGGEEHHHHHB0G=GBDDG0CBD DFGHHB/:C; @M03132:284:000000000-KM9YY:1:1101:18065:2943 1:N:0:ACGACTTG+AGCCGTAA GGTACTAGTTGGACAGTGTATCCCCCTTTATCAGGAAGTTATTCTCATTCGGGTCCTGCAGTAGATATTGCTATTTTTTCTTTACATTTAGCTGGTATATCTTCAATAGCTGGAGCA ATAAATTTTATAGTTACAATATTTAATATGCGTTGTAGAGGTTTATCTTTAGATATACTTCCTTTATTTTGTTGAGCTGTTTTATATACAGCATTTTTATTATTATTATCTTTACCT GTTTTAGCTGGTGCTAT + AAAAAFFFFFFFGGGGGGGGGGHHGGGHHHHHHHHHHHHHHHHHHHHHHHHHGHGHHIHHHHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHGHHHHHGHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHGHHGHHHHHHHHHHHHGEEEFEHHHHBGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHGHHHHHHHHHHHGHHHFFHH HHHHHHBFHFFGBGHFH
and R2_p:
@M03132:284:000000000-KM9YY:1:1101:18065:2943 2:N:0:ACGACTTG+AGCCGTAA TAGACTTCGGGATGGCCAAAGAATCAAAATAAATGTTGATACAATATAGGGTCTCCACCACCTTCAGGTTCAAAAAATGTTGTACTAACATTTCTATCAGTTAATAACATAGTAATA GCACCAGCTAAAAC +
Many thanks in advance.