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What is the extra 1 base in the Truseq adapter file (TruSeq3-PE-2.fa) #58

Open wjlim opened 9 months ago

wjlim commented 9 months ago

I found that the adapter sequence you guys provided have the extra 1base compared with Illumina protocol.

TruSeq3-PE-2.fa

PrefixPE/1 TACACTCTTTCCCTACACGACGCTCTTCCGATCT PrefixPE/2 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT PE1 TACACTCTTTCCCTACACGACGCTCTTCCGATCT PE1_rc AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA PE2 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT PE2_rc AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC

Read1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Read2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

What is purpose of the extra base?