usadellab / Trimmomatic

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Nextera Adapters sequences. #63

Open Kauthar-Omar opened 8 months ago

Kauthar-Omar commented 8 months ago

For my research project we are trying to under why the full adapter sequences were provided for paired end adapter trimming (adapters/NexteraPE-PE.fa) instead of partial the adapter sequences (i.e. CTGTCTCTTATACACATCT+AGATGTGTATAAGAGACAG) as recommended by Illumina. Indeed 2% more reads are trimmed using adapters/NexteraPE-PE.fa file.