Sample Collection Chapter

vallenderlab / protocols

A repository for the protocols and guides used in Dr. Eric Vallender's lab.

https://vallenderlab.github.io/protocols/

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Sample Collection Chapter #13

Open grabear opened 5 years ago

grabear commented 5 years ago

I've added the Sample Collection chapter. It is currently just a place holder, but I'm going to add information about collecting samples for DNA, RNA, and microbiome (maybe). @sdhutchins I'm also gonna add some place holder section for scRNAseq and cell culture (fibroblasts).

It might also be good to touch on the process that I had to go through to get the rhesus blood, and to have access to the animal core (e.g. IACUC training and informed consent form for each protocol that you are on).

sdhutchins commented 5 years ago

Great idea! Maybe it could be Sample Collection and Preparation.

We can add in the prep for the saliva samples for NGS as well as add that protocol and add in prep for Sanger Sequencing that we've done for the DRD4 project.

grabear commented 5 years ago

Sample types:

[ ] Rhesus/Rat/Human Brain (fatty)
[ ] Rhesus Blood; Leukocytes
[ ] Rat Liver
[ ] Rhesus Feces
[ ] Human/Rhesus Saliva
[ ] Cell Culture
[ ] Rhesus Skin Biopsy

Sample Purpose:

[ ] iPSC generation
[ ] scRNAseq
[x] 16s rRNA (microbiome sequencing)
[ ] iPSC Creation
[x] DNA isolation and genotyping
[x] RNA isolation and phenotyping
[ ] Validation

...add in prep for Sanger Sequencing that we've done for the DRD4 project.

I think this should go in a Gene Profile chapter (or maybe in the appendix). But I definitely think it should be private until this info is published.

sdhutchins commented 5 years ago

I've looked over that a few times, and I think it's a very good list. Can't think of anything else.

For the sanger sequencing, I meant sticking just to that company's protocol (Can't think of the name).