vastgroup / vast-tools

A toolset for profiling alternative splicing events in RNA-Seq data.
MIT License
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Error with psiplot #97

Closed juliana-chu closed 2 years ago

juliana-chu commented 3 years ago

Hello, there!

I have currently experiencing an error with PSI-Plot. When I command "vast-tools plot /vast_out/cRPKM-araTha10-1.tab", it returns with an error saying that I don't have psiplot. The Error is: Trying to load required package: optparse Trying to load required package: psiplot Error: package or namespace load failed for ‘psiplot’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): namespace ‘rlang’ 0.4.5 is being loaded, but >= 0.4.7 is required psiplot did not load correctly! Now trying to install.. Loading required package: psiplot Error in loadPackages(c("psiplot", "methods", "labeling"), local.lib = paste(c(scriptPath, : quitting!!! I could not install psiplot for you! In addition: Warning messages: 1: package ‘psiplot’ is not available (for R version 3.5.1) 2: In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE, : there is no package called ‘psiplot’ Execution halted

I wonder how can I download psiplot into my computer? I have used this command before, but it ran completely fine. I don't know what happened with this time. Please help me! Thanks

mirimia commented 3 years ago

Hello!

It seems it's "just" an R package installation issue. Did you run it in the same computer before and it worked? Or was it in a different one?

I think the easiest is that you ask around for basic R help, since I have little idea about it. I think it should be easy to solve for someone with basic R knowledge, though. It seems to me that the automatic installation has probably changed for newer R versions, so it crashes. I'll also try to dig from my side.

I'm CCing @kcha and @UBrau, but I'm not sure they'll have time to help any time soon...

Thanks Manu

kcha commented 3 years ago

The error indicates that psiplot isn't installed. If it was working before, then perhaps check if you are using the same environment.

You can install psiplot using instructions here: https://github.com/kcha/psiplot#installation. Or perhaps better to run the provided install.R script in case you are missing any other dependencies.

Having said that, it looks like there is a bug in the vast-tools method that attempts to install missing R packages that are not hosted on CRAN. I don't have time to look at it now but easiest solution is to ensure that the prerequisites have been installed (manually or via install.R).

mirimia commented 3 years ago

Thanks a lot @kcha !

RE the bug: I think it's just that the vast-tools installer got obsolete for new R versions. That method worked for old versions. We'll try to fix it.

M

juliana-chu commented 3 years ago

Thanks for the advices!

I have tried to reload the R packages, but it still returns errors. These are the errors I've got from Scient: Warning in install.packages(c("optparse", "RColorBrewer", "reshape2", "ggplot2", : 'lib = "/gpfs/fs1/scinet/niagara/software/2019b/opt/gcc-8.3.0/r/3.5.3/lib64/R/library"' is not writable Error in install.packages(c("optparse", "RColorBrewer", "reshape2", "ggplot2", : unable to install packages Error in loadNamespace(name) : there is no package called ‘devtools’ Calls: :: ... tryCatch -> tryCatchList -> tryCatchOne -> <Anonymous> Execution halted And these are the errors I've got from my local terminal: > install.packages(c("optparse", "RColorBrewer", "reshape2", "ggplot2", "devtools")) Error in contrib.url(repos, type) : trying to use CRAN without setting a mirror Calls: install.packages -> grep -> contrib.url Execution halted

Please tell me how to correct it.

Thanks, Juliana

kcha commented 3 years ago

The first error seems like you don't have the correct permissions to install R packages on Scient. Should probably ask your admin.

The second error is saying that you need to set a mirror. install.packages(..., repos='http://cran.us.r-project.org')

Did using the install.R script not work?

juliana-chu commented 3 years ago

Hello, sorry for the late reply.

I used install.R but it still didn't work, but I have reload my R into version 3.5. Then it worked!

Thank you for all!