I was wondering on the data that are passed to Skyline Daily after DIANN analysis. I was able to import the result.tsv thanks to another thread here (number 1048, thanks!), but I wanted to ask what I am looking at, when I see the results from DIANN into skyline.
I see the library that DIANN built and the raw files read on this library by skyline, correct? Are these raw files corrected by the peak boundaries? I ask this because when I import such peak boundaries from the report.tsv file, many precursors that were yellow/red turn into green in skyline, and I was wondering if this phenomenon was only aesthetical (with no changes in the quantification) or if importing the peak boundaries could actually help.
Thank you very much.
Best,
Giovanni
I was wondering on the data that are passed to Skyline Daily after DIANN analysis. I was able to import the result.tsv thanks to another thread here (number 1048, thanks!), but I wanted to ask what I am looking at, when I see the results from DIANN into skyline. I see the library that DIANN built and the raw files read on this library by skyline, correct? Are these raw files corrected by the peak boundaries? I ask this because when I import such peak boundaries from the report.tsv file, many precursors that were yellow/red turn into green in skyline, and I was wondering if this phenomenon was only aesthetical (with no changes in the quantification) or if importing the peak boundaries could actually help. Thank you very much. Best, Giovanni