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vdemichev / DiaNN

DIA-NN - a universal automated software suite for DIA proteomics data analysis.
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How to process lactylation proteomics data? #1071

Open ZexuanChenCN opened 5 months ago

ZexuanChenCN commented 5 months ago

We need to use DIA-NN to process our lactylation proteomics data, however we didn't find anywhere to input these parameter(lactylation modification or 72.021 Da Delta mass). So our project have to been paused.

Could you please add a parameter in your new released version?

Thanks, Best!

vdemichev commented 5 months ago

Please just add the correct —var-mod or —fixed-mod specification of the modification in Additional options.

ZexuanChenCN commented 4 months ago

Thanks for your answer，and is --var-mod Lac,72.021,K a correct command which as shown in the figure?（But software exited unfortunately, sad） Looking forward to hearing from you. Best!

微信图片_20240704152851

vdemichev commented 4 months ago

Please try DIA-NN 1.9 and the advice in the docs, i.e. first library generation and then analysis in the next pipeline step. I also recommend to enable peptidoform scoring in DIA-NN 1.9. The modification specification looks correct. The exit is due to some unrelated reason, i.e. could be lack of RAM or something wrong with the specific file.