As always, thank you for developing this excellent tool. We performed library-free mode search against a FASTA file and in the identified peptides, we found a few do not follow trypsin digestion rule. Specifically, it seems that the first start codon methionine is skipped (or cleaved?) for the peptides matched to the very beginning of the protein sequence, like an example shown below. Is this coded on purpose? Thanks a lot in advance for your answer.
Hi Vadim,
As always, thank you for developing this excellent tool. We performed library-free mode search against a FASTA file and in the identified peptides, we found a few do not follow trypsin digestion rule. Specifically, it seems that the first start codon methionine is skipped (or cleaved?) for the peptides matched to the very beginning of the protein sequence, like an example shown below. Is this coded on purpose? Thanks a lot in advance for your answer.
M
WIDLWLK
FVBest, Lili