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vdemichev / DiaNN

DIA-NN - a universal automated software suite for DIA proteomics data analysis.
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data normalization #886

Open fei2805 opened 11 months ago

fei2805 commented 11 months ago

When using DIA-NN to analyze multiple DIA mass spectrometry data simultaneously, how should the parameters be configured for data normalization?

vdemichev commented 11 months ago

Data normalisation is automatic, so please just follow the guidelines here https://github.com/vdemichev/DiaNN?tab=readme-ov-file#getting-started

fei2805 commented 11 months ago

Thank you for your response. Is the intensity provided in the report (eg. report.pr_matrix.tsv, report.pg_matrix.tsv) LFQ intensity, and can it be directly compared quantitatively? When conducting differential analysis (upregulation/downregulation), is there a need for additional correction of the intensity results?

vdemichev commented 11 months ago

Can use as is

fei2805 commented 11 months ago

Can use as is

Thanks a lot！