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vdemichev / DiaNN

DIA-NN - a universal automated software suite for DIA proteomics data analysis.
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installed dia_nn on to debian 12 with python3.9 #948

Open hrani opened 8 months ago

hrani commented 8 months ago

installed dia_nn via wget https://github.com/vdemichev/DiaNN/releases/download/1.8/diann_1.8.deb dpkg -i diann_1.8.deb /usr/diann/1.8/diann-1.8 --f 1.raw --f 2.raw --f 3.raw --f 4_UT.raw --lib "" --threads 4 --verbose 3 --out /home/report.tsv --qvalue 0.01 --matrices --out-lib /home/Data_Task\MHC_I_II_uniprot_predicted_library.tsv --gen-spec-lib --predictor --fasta /home/uniprotkb_proteome_UP000000589_2024_01_18.fasta --fasta-search --min-fr-mz 200 --max-fr-mz 1800 --met-excision --cut K,R we are getting Thermo RAW file format not supported. No MS2 spectra: aborting

This is on debian12/linux, what should be the library to be install in linux which can read this raw file

Thank you

vdemichev commented 8 months ago

Thermo RAW files are not supported by the native Linux build. Can either

Use Windows Use Wine 6.8 or later and install Thermo MSFileReader (specifically the version by the link in DIA-NN docs) under it Convert RAW to mzML or (under Windows) to .dia