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velocyto-team / velocyto.py

RNA velocity estimation in Python
http://velocyto.org/velocyto.py/
BSD 2-Clause "Simplified" License
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question about dropest_bc_correct #141

Open szimmerman92 opened 5 years ago

szimmerman92 commented 5 years ago

Hi,

I have been following the run_dropest tutorial at http://velocyto.org/velocyto.py/tutorial/cli.html#run-dropest-run-on-dropseq-indrops-and-other-techniques, but when I got to the "dropest_bc_correct" step I am receiving the error below.

KeyError: "tag 'CB' not present"

I looked at my BAM file outputted from dropest and it only has "CR" tags but not "CB" tags, indicating that I have raw cell barcodes only. Or at least that is what I think the "CR" tag means.

Have I made a mistake in some initial steps of the analysis?

I am using the using an in-drop dataset. Is there any more information I can supply that would be helpful?

Thank you.

kaplinserm commented 4 years ago

I have the same problem, did you solve it ?

szimmerman92 commented 4 years ago

sorry, I don't remember.