I looked at my BAM file outputted from dropest and it only has "CR" tags but not "CB" tags, indicating that I have raw cell barcodes only. Or at least that is what I think the "CR" tag means.
Have I made a mistake in some initial steps of the analysis?
I am using the using an in-drop dataset. Is there any more information I can supply that would be helpful?
Hi,
I have been following the run_dropest tutorial at http://velocyto.org/velocyto.py/tutorial/cli.html#run-dropest-run-on-dropseq-indrops-and-other-techniques, but when I got to the "dropest_bc_correct" step I am receiving the error below.
KeyError: "tag 'CB' not present"
I looked at my BAM file outputted from dropest and it only has "CR" tags but not "CB" tags, indicating that I have raw cell barcodes only. Or at least that is what I think the "CR" tag means.
Have I made a mistake in some initial steps of the analysis?
I am using the using an in-drop dataset. Is there any more information I can supply that would be helpful?
Thank you.