I am trying to follow the methods section of the paper "RNA velocity of single cells". In the method section, authors have written
For the 10x genomics platform datasets, the BAM file was processed using the default parameters of the Cellranger software (10x
Genomics).
Following this, I looked at the dataset GSE104323 and all the SRA files corresponding to P0 mouse. Then converted to FASTQ file using fastq-dump, generated genome index using the command
I am trying to follow the methods section of the paper "RNA velocity of single cells". In the method section, authors have written
Following this, I looked at the dataset GSE104323 and all the SRA files corresponding to P0 mouse. Then converted to FASTQ file using
fastq-dump
, generated genome index using the commandand then converted to bam file using the command
After that, I am not really sure what to do next. I am unable to follow
statement.
I tried a couple of things more:
I used fastq-dump to generate zipped fastq files as follows:
then renamed SRR6084365_1 to SRR6084365_1_S1_L001_R1_001.fastq.gz as described in another online article https://bioinformaticsworkbook.org/dataAnalysis/RNA-Seq/Single_Cell_RNAseq/Chromium_Cell_Ranger.html#gsc.tab=0 and downloaded mm10_rmsk.gtf as described in https://labs.wsu.edu/winuthayanon/basic-analysis-of-single-cell-rna-seq-data/how-to-analyze-single%E2%80%90cell-rna%E2%80%90seq/explaining-velocyto-command-line/
then I ran following:
then it generated a folder with name SRR6084_series
after that, I ran following velocyto command:
for which I am getting following error:
Clearly, I don't understand the step authors are referencing. So any help in this regard will be really helpful.
Thanks.