velocyto-team / velocyto.py

RNA velocity estimation in Python
http://velocyto.org/velocyto.py/
BSD 2-Clause "Simplified" License
159 stars 84 forks source link

Get tons of warnings when running #376

Open dingyaozhang opened 12 months ago

dingyaozhang commented 12 months ago

When I tried to run your software, I got tons of warnings, but I do not know why it happens: 2023-10-22 22:23:04,235 - DEBUG - Counting for batch 24536, containing 100 cells and 183 reads 2023-10-22 22:23:04,884 - DEBUG - 0 reads not considered because fully enclosed in repeat masked regions 2023-10-22 22:23:04,889 - WARNING - The barcode selection mode is off, no cell events will be identified by <80 counts 2023-10-22 22:23:04,895 - WARNING - 100 of the barcodes where without cell 2023-10-22 22:23:04,897 - DEBUG - Counting for batch 24537, containing 100 cells and 207 reads 2023-10-22 22:23:05,530 - DEBUG - 0 reads not considered because fully enclosed in repeat masked regions 2023-10-22 22:23:05,533 - WARNING - The barcode selection mode is off, no cell events will be identified by <80 counts 2023-10-22 22:23:05,540 - WARNING - 100 of the barcodes where without cell 2023-10-22 22:23:05,541 - DEBUG - Counting for batch 24538, containing 100 cells and 185 reads 2023-10-22 22:23:06,188 - DEBUG - 0 reads not considered because fully enclosed in repeat masked regions 2023-10-22 22:23:06,191 - WARNING - The barcode selection mode is off, no cell events will be identified by <80 counts 2023-10-22 22:23:06,197 - WARNING - 100 of the barcodes where without cell 2023-10-22 22:23:06,199 - DEBUG - Counting for batch 24539, containing 100 cells and 156 reads 2023-10-22 22:23:06,735 - DEBUG - 0 reads not considered because fully enclosed in repeat masked regions 2023-10-22 22:23:06,739 - WARNING - The barcode selection mode is off, no cell events will be identified by <80 counts 2023-10-22 22:23:06,745 - WARNING - 100 of the barcodes where without cell 2023-10-22 22:23:06,746 - DEBUG - Counting for batch 24540, containing 100 cells and 122 reads 2023-10-22 22:23:07,296 - DEBUG - 0 reads not considered because fully enclosed in repeat masked regions 2023-10-22 22:23:07,299 - WARNING - The barcode selection mode is off, no cell events will be identified by <80 counts 2023-10-22 22:23:07,305 - WARNING - 100 of the barcodes where without cell 2023-10-22 22:23:07,306 - DEBUG - Counting for batch 24541, containing 100 cells and 116 reads 2023-10-22 22:23:07,838 - DEBUG - 0 reads not considered because fully enclosed in repeat masked regions 2023-10-22 22:23:07,841 - WARNING - The barcode selection mode is off, no cell events will be identified by <80 counts 2023-10-22 22:23:07,847 - WARNING - 100 of the barcodes where without cell 2023-10-22 22:23:07,848 - DEBUG - Counting for batch 24542, containing 100 cells and 123 reads 2023-10-22 22:23:08,364 - DEBUG - 0 reads not considered because fully enclosed in repeat masked regions 2023-10-22 22:23:08,368 - WARNING - The barcode selection mode is off, no cell events will be identified by <80 counts 2023-10-22 22:23:08,374 - WARNING - 100 of the barcodes where without cell 2023-10-22 22:23:08,375 - DEBUG - Counting for batch 24543, containing 100 cells and 103 reads 2023-10-22 22:23:08,869 - DEBUG - 0 reads not considered because fully enclosed in repeat masked regions 2023-10-22 22:23:08,872 - WARNING - The barcode selection mode is off, no cell events will be identified by <80 counts 2023-10-22 22:23:08,879 - WARNING - 100 of the barcodes where without cell 2023-10-22 22:23:08,879 - DEBUG - Counting for batch 24544, containing 100 cells and 103 reads

Do you have any senses on what happened? Thank you very much in advance!!!!!

HelloWorldLTY commented 7 months ago

Same question here. Did you have any outputs?