I want to use ENSEMBL annotations because my CellRanger output was aligned using GRCh38 v 32 (Ensembl 98).
For that matter, I have the fasta sequence of the corresponding release, Homo_sapiens.GRCh38.dna_rm.toplevel.fa.gz, in which the repeat sequences were hard-masked by the Ensembl consortium themselves.
But to use it as an input for velocyto, I need to massage it into a gtf file. How should I do this?
I want to use ENSEMBL annotations because my CellRanger output was aligned using GRCh38 v 32 (Ensembl 98).
For that matter, I have the fasta sequence of the corresponding release, Homo_sapiens.GRCh38.dna_rm.toplevel.fa.gz, in which the repeat sequences were hard-masked by the Ensembl consortium themselves.
But to use it as an input for velocyto, I need to massage it into a gtf file. How should I do this?