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vertesy
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pseudoBulk
Cluster-specific pseudo-bulk analysis of 10X single-cell RNA-seq data by connecting Seurat to the VBC RNA-seq pipeline.
https://vertesy.github.io/pseudoBulk
MIT License
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Implement subset-bam and pseudo-bulk QC
#2
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vertesy
opened
4 years ago
vertesy
commented
4 years ago
Steps
[x] Export barcodes in right format
[x] Run
subset-bam
[x] run VBC RNA-seq pipeline
vertesy
commented
4 years ago
Some questions are still open:
[ ] What happens with UMI duplicates / PCR duplicates
[ ] What happens with the UMI + cell barcodes - are they cut off?
Steps