Closed RohitKapila closed 3 months ago
vg can be quite complicated to use, but there is a fair bit of documentation and examples on the wiki, along with some basic information in the README. Please take a look there, to see that you need to index before mapping (previous issue) or that vg mpmap
does not output GAM by default (this issue). Also, it wouldn't hurt to update to a current vg release either.
1. What were you trying to do?
2. What did you want to happen?
3. What actually happened?
4. If you got a line like
Stack trace path: /somewhere/on/your/computer/stacktrace.txt
, please copy-paste the contents of that file here:5. What data and command can the vg dev team use to make the problem happen?
vg construct -f -S -I insertions.fa -a -r N2_refrence.fna -v N2_2Formal_Manta.vcf > 6graph.vg vg convert 6graph.vg -a > 6graph.gbz vg index 6graph.vg -L -x 6graph.xg vg index -x 6graph.xg -g 6graph.gcsa -k 16 6graph.vg vg mpmap -x 6graph.xg -g 6graph.gcsa -f N2_2Formal_Combined.fq > 6graph.gam vg snarls 6graph.gbz > 6graph.snarls vg pack -x 6graph.gbz -g 6graph.gam -o 6graph.pack -Q 5
vg version v1.44.0 "Solara" Compiled with g++ (Ubuntu 9.4.0-1ubuntu1~20.04.1) 9.4.0 on Linux Linked against libstd++ 20210601 Built by anovak@octagon