vibansal / HapCUT2

software tools for haplotype assembly from sequence data
BSD 2-Clause "Simplified" License
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Outdated README #91

Closed FrickTobias closed 4 years ago

FrickTobias commented 4 years ago

I think the README file could be a bit out of date and clarifying some things could hopefully reduce the number of issues posted having to do with user malpractice.

I've jotted down some things I've come across which to me seem like they could have been more true to an earlier version, or at least not clear enough for me to understand it fully.

vibansal commented 4 years ago

Many thanks for pointing out these issues. I have updated and cleaned up the README file. The phased output block file description has been moved to a separate file.

FrickTobias commented 4 years ago

Thanks a lot! Just one last thing I noticed, my files have 12 columns rather than the 11 which are documented.

 cat phase-file | head
BLOCK: offset: 261 len: 4231 phased: 2278 SPAN: 1886352 fragments 2645
261 -   -   chr1    789130  T   A   0/1:13:11,2:11:381:2:62:-0.087102,0,-19.4507    1   .   3.68    2
262 0   1   chr1    789445  T   A   0/1:12:10,2:10:370:2:50:-0.566989,0,-13.7097    0   .   26.22   3
265 1   0   chr1    790564  C   A   0/1:17:8,9:8:296:9:260:-1.4864,0,-12.391    0   .   100.00  2
268 1   0   chr1    792473  C   T   0/1:19:8,10:8:296:10:361:-0.160973,0,-12.8297   0   .   29.21   1
310 1   0   chr1    820143  C   T   0/1:7:5,2:5:159:2:74:-0.44739,0,-5.28299    0   .   35.51   2
311 1   0   chr1    820169  T   G   0/1:7:5,2:5:147:2:74:-0.162553,0,-7.48213   1   .   36.76   2
317 0   1   chr1    821264  A   T   0/1:14:12,2:12:406:2:74:-0.212649,0,-16.9445    1   .   22.46   2
318 0   1   chr1    822248  T   A   0/1:12:8,3:8:258:3:86:-0.662489,0,-12.0419  0   .   22.00   1
325 1   0   chr1    823414  C   G   0/1:14:12,2:12:432:2:74:-0.485248,0,-27.4007    0   .   100.00  4
vibansal commented 4 years ago

I will update this. Column 12 is the coverage of the variant in the fragment file (input to HapCUT2).

FrickTobias commented 4 years ago

Great, thanks!