Phase II Lab/Experiment Hosting/Re-hosting Request for Immunology Virtual Lab II - AU

[amritamindbrain]

1. Lab Name: Immunology Virtual Lab II

2. List of Experiments and Repositories:

Ouchterlony Double Diffusion -Titration
https://github.com/virtual-labs/exp-ouchterlony-double-diffusion-titration-au v1.0.0

Ouchterlony Double Diffusion - Patterns https://github.com/virtual-labs/exp-ouchterlony-double-diffusion-patterns-au v1.0.0

Purification of IgG Antibodies with Ammonium Sulphate https://github.com/virtual-labs/exp-ammonium-sulphate-au v1.0.0

Removal of Thymus and Spleen from Mice https://github.com/virtual-labs/exp-spleen-from-mice-au v1.0.0

Mouse Anesthesia and Blood Collection https://github.com/virtual-labs/exp-mouse-anesthesia-au v1.0.0

Parenteral Injections https://github.com/virtual-labs/exp-parenteral-injections-au v1.0.0

Purification of IgG Antibodies using Affinity Chromatography https://github.com/virtual-labs/exp-affinity-chromatography-au v1.0.0

Fluorescent Labeling of Antibodies https://github.com/virtual-labs/exp-fluorescent-labeling-of-antibodies-au v1.0.0

Fragmentation of IgG Using Papain https://github.com/virtual-labs/exp-igg-using-papain-au v1.0.0

Fragmentation of IgG using pepsin https://github.com/virtual-labs/exp-igg-using-pepsin-au v1.0.0

3. Course Alignment:

• UG in Biotechnology/Microbiology/Bioinformatics/Biomedical Science
• PG in Biotechnology/Microbiology/Bioinformatics/Biomedical Science
• B.Tech./ B.E in Biotechnology

4. Target Audience: (1) UG (2) PG (3) PhD/Research Scholars (4) B Tech (5) M Tech

5. Broad Area : Biotechnology and Biomedical Engineering

6. Introduction :

The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from no self, and laboratory techniques involving the interaction of antigens with specific antibodies.

7. Objective of the Lab:

• To introduce the principle of precipitation reactions and differentiate it from agglutination reactions
• To demonstrate the purification protocols of antibodies for use in research and diagnostics
• To understand the concepts of mouse Euthanasia. To learn the basic procedures involved in rodent dissection and how to identify and remove lymphoid organs.
• To understand the basic principles in fragmentation of IgG to a monovalent Fab fragment using Papain digestion. The cleavage of antibodies using the Proteolytic enzyme is first studied by porter in 1959. IgG is cleaved by Papain to give Fab fragments
• To understand the principles of IgG fragmentation to a divalent F(ab')2 fragment.

[pavanchow]

@amritamindbrain

Hosted Immunology Virtual Lab II with mentioned experiments URL of Lab: https://ivl2-au.vlabs.ac.in

Please check and approve

[amritamindbrain]

@pavanchow

rehost the 2 experiments listed below. rest everything is ok

Ouchterlony Double Diffusion -Titration https://github.com/virtual-labs/exp-ouchterlony-double-diffusion-titration-au v1.0.1

Fragmentation of IgG using pepsin https://github.com/virtual-labs/exp-igg-using-pepsin-au v1.0.1

[pavanchow]

@amritamindbrain

Hosted Immunology Virtual Lab II with mentioned experiments URL of Lab: https://ivl2-au.vlabs.ac.in

Please check and approve

[amritamindbrain]

@pavanchow

verified

[ravikiran2020]

Rehosting the lab with Bug Report and Rating Feature