Experiment Repository Creation Request for "Experimental Biochemistry" IIT Kharagpur

[prakriti5dhang]

Repository Creation Request

1. Coordinating Institute: Indian Institute of Technology Kharagpur

2. Approved Proposal: Experimental Biochemistry

3. Virtual Lab Name: Experimental Biochemistry

4. Experiment List:

   • Computation of various physical and chemical properties of Proteins and DNA from their primary sequence.
   • DNA amplification by polymerase chain reaction (PCR).
   • DNA analysis by agarose gel electrophoresis.
   • pH-titration of amino acids and small peptides, and estimation of isoelectric point (pI).
   • Protein analysis by polyacrylamide gel electrophoresis (PAGE).
   • Protein purification by affinity chromatography.
   • Protein purification by size exclusion chromatography (SEC).
   • Protein-DNA interaction analysis by electrophoretic mobility shift assay (EMSA).
   • Thermodynamic characterization of protein-ligand binding by isothermal titration calorimetry (ITC).
   • Kinetic characterization of protein-ligand binding by surface plasmon resonance (SPR).

5. Primary GitHub Handle Details:

   Name: Virtual Labs IIT Kharagpur

   GitHub Handle: @VLabs-IITKharagpur

   Email id: vlabsiitkgpaws@gmail.com

6. Secondary GitHub Handle Details:

   Name: Prakriti Dhang

   GitHub Handle: @prakriti5dhang

   Email id: prakritidhang@gmail.com

[ravikiran2020]

@virtual-labs/iitkgp Please find the links for Experimental Biochemistry Lab experiments and populate the repositories with sources of the experiments as detailed in Step 4 of the migration document.

1. Computation of various physical and chemical properties of Proteins and DNA from their primary sequence - https://github.com/virtual-labs/exp-physical-chemical-properties-proteins-dna-iitkgp
2. DNA amplification by polymerase chain reaction (PCR) - https://github.com/virtual-labs/exp-dna-pcr-iitkgp
3. DNA analysis by agarose gel electrophoresis - https://github.com/virtual-labs/exp-dna-agarose-gel-electrophoresis-iitkgp
4. pH-titration of amino acids and small peptides, and estimation of isoelectric point (pI) - https://github.com/virtual-labs/exp-ph-isoelectric-point-iitkgp
5. Protein analysis by polyacrylamide gel electrophoresis (PAGE) - https://github.com/virtual-labs/exp-protein-page-iitkgp
6. Protein purification by affinity chromatography - https://github.com/virtual-labs/exp-protein-chromatography-iitkgp
7. Protein purification by size exclusion chromatography (SEC) - https://github.com/virtual-labs/exp-protein-sec-iitkgp
8. Protein-DNA interaction analysis by electrophoretic mobility shift assay (EMSA) -https://github.com/virtual-labs/exp-protein-dna-emsa-iitkgp
9. Thermodynamic characterization of protein-ligand binding by isothermal titration calorimetry (ITC) - https://github.com/virtual-labs/exp-thermodynamic-itc-iitkgp
10. Kinetic characterization of protein-ligand binding by surface plasmon resonance (SPR) - https://github.com/virtual-labs/exp-protein-ligand-spr-iitkgp

[prakriti5dhang]

Thank you @ravikiran2020 for creating the repositories. One observation: exp 1 and exp 4 links are not working in the About section.

[ravikiran2020]

@prakriti5dhang Can you please check them now.

[prakriti5dhang]

Yes, the links are working now. Thank you.