Open lulunisrna opened 4 years ago
Hi, I've also been having a hard time finding suitable parameters for my soil datasets (#254). Did you manage to improve your N50 somehow?
Soil samples are hard to assemble because of
No solution to the first problem other than sequencing a lot more data. For the second problem normalization may help. See https://github.com/voutcn/megahit/issues/239#issuecomment-534373589
Dear voutcn,
I have problem with my result for using MEGAHIT. My result for N50 is very short, around 450-550bp. My sample is from soil plantation. I have reed the same issue from this page and you give an advise for using min
--min-count 1
, but it doesn't work for me. I also already tried to running assemblywith --kmin-1pass
, but the result of N50 also too short, around 500bp. Now i'm trying for using--presets meta-large
for this assembly. I hope i will get the good result. If my result is still bad, do you have an advice for me to fix this problem? Thank you.