voutcn / megahit

Ultra-fast and memory-efficient (meta-)genome assembler
http://www.ncbi.nlm.nih.gov/pubmed/25609793
GNU General Public License v3.0
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different assemble runing megahit with same sample and same parameters #264

Closed feihongloveworld closed 4 years ago

feihongloveworld commented 4 years ago

i run many times for a sample with megahit,but got different assmble fasta ....... What does make the difference ? cmd as below:

megahit -r R1.fastq -o xxxx --out-prefix xxxx or megahit -1 R1.fastq -2 R2.fastq -o xxxx --out-prefix xxxx

fconstancias commented 4 years ago

megahit -r R1.fastq -o xxxx --out-prefix xxxx : you are using only R1 megahit -1 R1.fastq -2 R2.fastq -o xxxx --out-prefix xxxx : you are using both R1 and R2 of your pair-end librairy