Closed feihongloveworld closed 4 years ago
i run many times for a sample with megahit,but got different assmble fasta ....... What does make the difference ? cmd as below:
megahit -r R1.fastq -o xxxx --out-prefix xxxx or megahit -1 R1.fastq -2 R2.fastq -o xxxx --out-prefix xxxx
megahit -r R1.fastq -o xxxx --out-prefix xxxx : you are using only R1 megahit -1 R1.fastq -2 R2.fastq -o xxxx --out-prefix xxxx : you are using both R1 and R2 of your pair-end librairy
megahit -r R1.fastq -o xxxx --out-prefix xxxx
megahit -1 R1.fastq -2 R2.fastq -o xxxx --out-prefix xxxx
i run many times for a sample with megahit,but got different assmble fasta ....... What does make the difference ? cmd as below:
megahit -r R1.fastq -o xxxx --out-prefix xxxx or megahit -1 R1.fastq -2 R2.fastq -o xxxx --out-prefix xxxx