When I run the command, Megahit can only recognize one fq file, what happens

[chenyu-11]

command:megahit -r R1_L_Unaligned.fq, R2_L_Unaligned.fq, R1_L_alignedmate.fq, R2_L_alignedmate.fq, -o L.fa the process is as follows: 2022-09-13 00:39:25 - MEGAHIT v1.2.9 2022-09-13 00:39:25 - Using megahit_core with POPCNT and BMI2 support 2022-09-13 00:39:25 - Convert reads to binary library 2022-09-13 00:39:25 - b'INFO sequence/io/sequence_lib.cpp : 75 - Lib 0 (/home/yfli/gy/map/unmap/R1_L_Unaligned.fq): se, 47956 reads, 150 max length' 2022-09-13 00:39:25 - b'INFO utils/utils.h : 152 - Real: 0.0590\tuser: 0.0496\tsys: 0.0114\tmaxrss: 9308' 2022-09-13 00:39:25 - k-max reset to: 141 2022-09-13 00:39:25 - Start assembly. Number of CPU threads 384 2022-09-13 00:39:25 - k list: 21,29,39,59,79,99,119,141 2022-09-13 00:39:25 - Memory used: 2191223483596 2022-09-13 00:39:25 - Extract solid (k+1)-mers for k = 21 2022-09-13 00:39:27 - Build graph for k = 21 2022-09-13 00:39:32 - Assemble contigs from SdBG for k = 21 2022-09-13 00:39:35 - Local assembly for k = 21 2022-09-13 00:39:40 - Extract iterative edges from k = 21 to 29 2022-09-13 00:39:41 - Build graph for k = 29 2022-09-13 00:39:42 - Assemble contigs from SdBG for k = 29 2022-09-13 00:39:44 - Local assembly for k = 29 2022-09-13 00:39:49 - Extract iterative edges from k = 29 to 39 2022-09-13 00:39:49 - Build graph for k = 39 2022-09-13 00:39:50 - Assemble contigs from SdBG for k = 39 2022-09-13 00:39:51 - Local assembly for k = 39 2022-09-13 00:39:55 - Extract iterative edges from k = 39 to 59 2022-09-13 00:39:56 - Build graph for k = 59 2022-09-13 00:39:57 - Assemble contigs from SdBG for k = 59 2022-09-13 00:39:58 - Local assembly for k = 59 2022-09-13 00:40:03 - Extract iterative edges from k = 59 to 79 2022-09-13 00:40:03 - Build graph for k = 79 2022-09-13 00:40:04 - Assemble contigs from SdBG for k = 79 2022-09-13 00:40:06 - Local assembly for k = 79 2022-09-13 00:40:10 - Extract iterative edges from k = 79 to 99 2022-09-13 00:40:10 - Build graph for k = 99 2022-09-13 00:40:11 - Assemble contigs from SdBG for k = 99 2022-09-13 00:40:12 - Local assembly for k = 99 2022-09-13 00:40:16 - Extract iterative edges from k = 99 to 119 2022-09-13 00:40:16 - Build graph for k = 119 2022-09-13 00:40:17 - Assemble contigs from SdBG for k = 119 2022-09-13 00:40:18 - Local assembly for k = 119 2022-09-13 00:40:23 - Extract iterative edges from k = 119 to 141 2022-09-13 00:40:23 - Build graph for k = 141 2022-09-13 00:40:24 - Assemble contigs from SdBG for k = 141 2022-09-13 00:40:24 - Merging to output final contigs 2022-09-13 00:40:24 - 1575 contigs, total 1059403 bp, min 301 bp, max 3688 bp, avg 672 bp, N50 745 bp 2022-09-13 00:40:24 - ALL DONE. Time elapsed: 59.210746 seconds