Hi~I rerun metaspades in a new machine, which memory has 512G, indeed, it worked, but I meet another error "Sorry, current version of metaSPAdes can work either with single library (paired-end only) or in hybrid paired-end + (TSLR or PacBio or Nanopore) mode."
Is metaSPADES cannot assemble multiple sets of paired-end sequences? Can I solve this problem by merge all paired-end sequences into one sequence file?
my input code: time spades.py --meta -o /project01/workspace/Metagenome/R1rawdata/result/metaspades --pe1-1 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.224d.R1.paired.fq.gz --pe2-1 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.496d.R1.paired.fq.gz --pe3-1 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.590d.10G.R1.paired.fq.gz --pe1-2 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.224d.R2.paired.fq.gz --pe2-2 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.496d.R2.paired.fq.gz --pe3-2 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.590d.10G.R2.paired.fq.gz -k 21,33,55,77,99,127 -m 400 -t 20 --phred-offset 33
Hi~I rerun metaspades in a new machine, which memory has 512G, indeed, it worked, but I meet another error "Sorry, current version of metaSPAdes can work either with single library (paired-end only) or in hybrid paired-end + (TSLR or PacBio or Nanopore) mode." Is metaSPADES cannot assemble multiple sets of paired-end sequences? Can I solve this problem by merge all paired-end sequences into one sequence file? my input code: time spades.py --meta -o /project01/workspace/Metagenome/R1rawdata/result/metaspades --pe1-1 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.224d.R1.paired.fq.gz --pe2-1 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.496d.R1.paired.fq.gz --pe3-1 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.590d.10G.R1.paired.fq.gz --pe1-2 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.224d.R2.paired.fq.gz --pe2-2 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.496d.R2.paired.fq.gz --pe3-2 /project01/workspace/Metagenome/R1rawdata/result/trimmomatic/R1.590d.10G.R2.paired.fq.gz -k 21,33,55,77,99,127 -m 400 -t 20 --phred-offset 33