Error: Exit code -7

[MoriatyZy]

Hello Voutcn,

I got his error today on an assembly (I applied for 256G of memory space):

2023-10-20 19:58:03 - Error occurs, please refer to /gpfs/share/home/1700013510/OtherData/RiverTl/temp/megahit/log for detail 2023-10-20 19:58:03 - Command: /gpfs/share/home/1700013510/anaconda3/envs/meta_env/bin/megahit_core assemble -s /gpfs/share/home/1700013510/OtherData/RiverTl/temp/megahit/tmp/k21/21 -o /gpfs/share/home/1700013510/OtherData/RiverTl/temp/megahit/intermediate_contigs/k21 -t 32 --min_standalone 300 --prune_level 2 --merge_len 20 --merge_similar 0.95 --cleaning_rounds 5 --disconnect_ratio 0.1 --low_local_ratio 0.2 --cleaning_rounds 5 --min_depth 2 --bubble_level 2 --max_tip_len -1 --careful_bubble; Exit code -7

I wonder if this is a memory issue or something else? If it's because of insufficient disk space, how much memory should I request? I have 6 paired samples (that means 12 files--fq.gz), and each is about 5GB. I'd appreciate if you tell me how much running memory and threads I need! In addition, I would like to know if I can continue assembling on the basis of the previous run stop, instead of starting everything over.

Thank you so much again! The operation log and the input samples are attached here. log.txt