Hi,
I am getting the following error when I try to run Pamir:
Creating a new project folder... OK
Checking binary pre-requisites... ... OK
Sorting bam file... SKIPPING
Extracting FASTQ from Alignment file... SKIPPING
Masking Reference Genome... OK
Traceback (most recent call last):
File "/path/to/pamir/pamir.py", line 1448, in <module>
sys.exit(main())
File "/path/to/pamir/pamir.py", line 1438, in main
run_command(config)
File "/path/to/pamir/pamir.py", line 1005, in run_command
index(config)
File "/path/to/pamir/pamir.py", line 327, in index
cmd = pipeline.mrsfast + ' --index {0} --ws {1}'.format(input_file, config.get("mrsfast","window_size") )
AttributeError: class pipeline has no attribute 'mrsfast'
Can you tell what's going on here? Is the call valid? Input bams are name-sorted.
(Mabye important) Sidenote: The folder /path/to/workdir/sorted_bams/ does not really contain mappings from mrsFast but BWA. I was told ages ago that --files mrsfast-best-search can also handle BWA bam files, please correct me if I am wrong here.
I also tried a run with --files alignment=\<aln1>.bwa,\<aln2>.bwa,...,\<alnN>.bwa but that one never started. I am trying to call something like your README's example (2) or (3).
Hi, I am getting the following error when I try to run Pamir:
and indeed class pipeline has no such attribute.
My call was:
Can you tell what's going on here? Is the call valid? Input bams are name-sorted.
(Mabye important) Sidenote: The folder /path/to/workdir/sorted_bams/ does not really contain mappings from mrsFast but BWA. I was told ages ago that --files mrsfast-best-search can also handle BWA bam files, please correct me if I am wrong here.
I also tried a run with --files alignment=\<aln1>.bwa,\<aln2>.bwa,...,\<alnN>.bwa but that one never started. I am trying to call something like your README's example (2) or (3).
Thanks!