I am using SvABA for structural variant calling in a whole exome sequencing (WES) project. My dataset includes WES data from a family with three affected children, one unaffected child, and both parents. The data is not tumor/normal matched.
Problem:
I encountered an error when running SvABA with the following command:
svaba run -t /path/to/affected_child.bam -n /path/to/unaffected_child.bam -G /path/to/reference.fa -R /path/to/regions.bed -a output_prefix
Caugt error:stoi
ERROR: Cannot read region file: /path/to/regions.bed or something wrong with bam header ('chr' prefix mismatch?)
i checked that both of my exome capture bed file and reference file start with 'chr'
how can i solve this problem
thanks in advance
Hello SvABA Team,
I am using SvABA for structural variant calling in a whole exome sequencing (WES) project. My dataset includes WES data from a family with three affected children, one unaffected child, and both parents. The data is not tumor/normal matched.
Problem:
I encountered an error when running SvABA with the following command: svaba run -t /path/to/affected_child.bam -n /path/to/unaffected_child.bam -G /path/to/reference.fa -R /path/to/regions.bed -a output_prefix Caugt error:stoi ERROR: Cannot read region file: /path/to/regions.bed or something wrong with bam header ('chr' prefix mismatch?)
i checked that both of my exome capture bed file and reference file start with 'chr' how can i solve this problem thanks in advance