Hi there. I am trying to use exhaustive but encountered some issues. I have a micro.fna file and a human.fna file. I am trying to remove human contamination from the microbial sequences. I ran the following command:
python3 exhaustive.py submit -l demo -d human.fna -b human_bt2 -f micro_file.txt
However, the output demo.contaminated.fna contains sequences from human.fna. This seems inconsistent with my expectations. Did I make a mistake with the code?
Additionally, can the .txt contain multiple directory paths? If so, what is the operational logic for this?
Thank you for your assistance!
Hi there. I am trying to use exhaustive but encountered some issues. I have a micro.fna file and a human.fna file. I am trying to remove human contamination from the microbial sequences. I ran the following command:
python3 exhaustive.py submit -l demo -d human.fna -b human_bt2 -f micro_file.txtHowever, the output demo.contaminated.fna contains sequences from human.fna. This seems inconsistent with my expectations. Did I make a mistake with the code?Additionally, can the .txt contain multiple directory paths? If so, what is the operational logic for this? Thank you for your assistance!