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wdecoster / NanoPlot

Plotting scripts for long read sequencing data
http://nanoplot.bioinf.be
MIT License
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ERROR: expected columns in summary file sequencing_summary.txt not found after updates of minKNOW #240

Closed stevebaeyen closed 3 years ago

stevebaeyen commented 3 years ago

after this command: ` afbeelding, I get this error:

afbeelding the first row is: filename_fastq filename_fast5 read_id run_id channel mux start_time duration pore_type experiment_id sample_id end_reason

wdecoster commented 3 years ago

Confusingly, there are two types of sequencing summary files created. This is one generated during the actual sequencing but does not include information about the basecalling (read lengths, qualities).

stevebaeyen commented 3 years ago

Thanks for your fast reply! Indeed, the basecalling itself was done on another PC than the one used for sequencing... will search for the good file. In the meantime, i ran NanoPlot on the fastq files.