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wdecoster / NanoPlot

Plotting scripts for long read sequencing data
http://nanoplot.bioinf.be
MIT License
407 stars 48 forks source link

Nanoplot histgrams has minus bars #288

Closed khoriba closed 2 years ago

khoriba commented 2 years ago

Hi, I used Nanoplot for my MinION seq data as follows. $ NanoPlot --fastq B01.fq --loglength -t 8 -o B01_np Then I got histgrams with minus bars in "weight histogram of read length" and "weight histogram of read length after log transformation". This Nanoplot was installed using miniconda. Afterward, the version of seaborn was dropped to 0.10.0 to avoid errors. The other nanopore output data is outputting "normally". What are the possible reasons? b01

wdecoster commented 2 years ago

This is most unexpected. Can you tell me which version of NanoPlot you have? It seems outdated, as current plots are produced with plotly and look a bit different.

khoriba commented 2 years ago

Thank you for your reply.

$ NanoPlot -v NanoPlot 1.20.0

wdecoster commented 2 years ago

The most recent version on PyPI (pip) and conda is v1.39.0, could you try upgrading?

khoriba commented 2 years ago

I finally got the latest version. Unfortunately, I couldn't get the latest Nanoplot without specifying the version. It looks like a normal graph has been created with v1.39.0. Thank you!! WeightedHistogramReadlength !

wdecoster commented 2 years ago

Thank you for the feedback!