Hi bro,
I am using NanoPlot 1.40.0 to look nanopore data (CRR302667.fastq.gz, downloaded from https://www.sciencedirect.com/science/article/pii/S1672022921001741).
My sever is debian system 64bit, and my command is :
nohup NanoPlot -t 10 --fastq ../CRR302667.fastq.gz --plots hex dot kde -o 01.Nanoplot -p Ath -cm Viridis &
Finally, I am confused, and how could I intepreted the following parts (or am I missed something parameters of NanoPlot):
(1) my results did not include hex png file,
(2) and I also found the statistics summary was somehow different, and I was confused the quality >Q15: 6640 (0.2%) 12.3Mb was really?
(3) and Top 5 highest mean basecall quality scores and their read lengths read length is 1?
AthNanoStats.txt content:
General summary:
Mean read length: 18,541.3
Mean read quality: 11.1
Median read length: 7,818.0
Median read quality: 11.2
Number of reads: 3,064,191.0
Read length N50: 46,452.0
STDEV read length: 26,536.0
Total bases: 56,814,196,989.0
Number, percentage and megabases of reads above quality cutoffs
>Q5: 3064191 (100.0%) 56814.2Mb
>Q7: 3064123 (100.0%) 56814.2Mb
>Q10: 2168595 (70.8%) 40456.1Mb
>Q12: 1055916 (34.5%) 19383.7Mb
>Q15: 6640 (0.2%) 12.3Mb
Top 5 highest mean basecall quality scores and their read lengths
1: 21.0 (1)
2: 19.0 (1)
3: 19.0 (1)
4: 19.0 (1)
5: 18.9 (358)
Top 5 longest reads and their mean basecall quality score
1: 495032 (12.4)
2: 457760 (8.7)
3: 439434 (9.1)
4: 438143 (8.7)
5: 431286 (9.7)
Hi bro, I am using
NanoPlot 1.40.0
to look nanopore data (CRR302667.fastq.gz, downloaded fromhttps://www.sciencedirect.com/science/article/pii/S1672022921001741
). My sever is debian system 64bit, and my command is :Finally, I am confused, and how could I intepreted the following parts (or am I missed something parameters of NanoPlot): (1) my results did not include hex png file, (2) and I also found the statistics summary was somehow different, and I was confused the quality
>Q15: 6640 (0.2%) 12.3Mb
was really? (3) andTop 5 highest mean basecall quality scores and their read lengths
read length is 1?AthNanoStats.txt content:
Thanks in advance,
From cfc424