How is mean Qscore calculated? Why is it different from the mean of the mean_qscore from the sequencing_summa

wdecoster / NanoPlot

Plotting scripts for long read sequencing data

http://nanoplot.bioinf.be

MIT License

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How is mean Qscore calculated? Why is it different from the mean of the mean_qscore from the sequencing_summa #327

Closed lgraciamaz closed 1 year ago

lgraciamaz commented 1 year ago

How is mean Qscore calculated and why is it different than the value you obtain from calculating the mean of the mean_qscore column from the sequencing_summary.txt file?

wdecoster commented 1 year ago

Means are calculated by converting Phred scores to error/accuracy probabilities, taking the mean, and converting back to the Phred scale.