welch-lab / liger

R package for integrating and analyzing multiple single-cell datasets
GNU General Public License v3.0
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Suggestions for quick merge (or integrate) scATAC replications #195

Closed wangmeijiao closed 3 years ago

wangmeijiao commented 4 years ago

Hi,

Unlike scRNA experiments, scATAC uses peak as features instead of genes, which can yield a much bigger dcgMatrix than scRNA. I understand how liger deal with scRNA + scATAC integration (transform peak x cell to gene_activity x cell). And I just wonder how to quick merge (or integrate) two or more scATAC replications as one with liger. Cellranger-atac aggre could be a solution, but this often leads to depth bias even the '--normalize=depth' was used.