I am trying to run some analysis where I have a seurat object with normalised expression matrix and raw expression matrix that are being used as input in marvel object creation along with splice junction files created with starSOLO.
What I want to do is to create a marvel object that has the information from all splice junction files corresponding to each sample. For instance, in the seurat object, I have 6 samples that are merged. Now, I want to create a single marvel object for this merged data such that I can infer the splicing pattern of these samples and do the same with another group of samples stored in another seurat object.
Is there a way to do that? Also, is there a way to identify which cell type does the splicing information correspond to? Because for gene expression, I have cell type specific seurat objects, and I intend to understand for instance how splicing differs in cell type 1 versus cell type 2 for the same transcript.
If you would need to have some data, I can subset my data and can share the object with you.
Thanks in advance for your help.
Hi,
I am trying to run some analysis where I have a seurat object with normalised expression matrix and raw expression matrix that are being used as input in marvel object creation along with splice junction files created with starSOLO. What I want to do is to create a marvel object that has the information from all splice junction files corresponding to each sample. For instance, in the seurat object, I have 6 samples that are merged. Now, I want to create a single marvel object for this merged data such that I can infer the splicing pattern of these samples and do the same with another group of samples stored in another seurat object. Is there a way to do that? Also, is there a way to identify which cell type does the splicing information correspond to? Because for gene expression, I have cell type specific seurat objects, and I intend to understand for instance how splicing differs in cell type 1 versus cell type 2 for the same transcript.
If you would need to have some data, I can subset my data and can share the object with you. Thanks in advance for your help.
Best, Rahul