Thank you for the nice tool.
We plan to analyze alternative splicing using MARVEL. However, Starsolo generated some wired results as shown in the below log file. The same code from https://wenweixiong.github.io/MARVEL_Droplet.html was used. Do you have any idea about the data preparation based on the GEX bam file of multiome data?
Thanks!
Log file:
Started job on | Oct 04 00:08:13
Started mapping on | Oct 04 00:08:30
Finished on | Oct 04 00:08:32
Mapping speed, Million of reads per hour | 0.00
Number of input reads | 0
Average input read length | 0
UNIQUE READS:
Uniquely mapped reads number | 0
Uniquely mapped reads % | 0.00%
Average mapped length | 0.00
Number of splices: Total | 0
Number of splices: Annotated (sjdb) | 0
Number of splices: GT/AG | 0
Number of splices: GC/AG | 0
Number of splices: AT/AC | 0
Number of splices: Non-canonical | 0
Mismatch rate per base, % | -nan%
Deletion rate per base | 0.00%
Deletion average length | 0.00
Insertion rate per base | 0.00%
Insertion average length | 0.00
MULTI-MAPPING READS:
Number of reads mapped to multiple loci | 0
% of reads mapped to multiple loci | 0.00%
Number of reads mapped to too many loci | 0
% of reads mapped to too many loci | 0.00%
UNMAPPED READS:
Number of reads unmapped: too many mismatches | 0
% of reads unmapped: too many mismatches | 0.00%
Number of reads unmapped: too short | 0
% of reads unmapped: too short | 0.00%
Number of reads unmapped: other | 0
% of reads unmapped: other | 0.00%
CHIMERIC READS:
Number of chimeric reads | 0
% of chimeric reads | 0.00%
ywzhang071394
commented
1 month ago
Hi Wenwei,
Thank you for the nice tool. We plan to analyze alternative splicing using MARVEL. However, Starsolo generated some wired results as shown in the below log file. The same code from https://wenweixiong.github.io/MARVEL_Droplet.html was used. Do you have any idea about the data preparation based on the GEX bam file of multiome data? Thanks!
Log file: