williamritchie / IRFinder

Detecting intron retention from RNA-Seq experiments
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this program suitable for non model organisn #106

Closed ucbtsh6 closed 4 years ago

ucbtsh6 commented 4 years ago

Im dealing with non model organism its genome doesn't exists on ENsemble but I have GTF file with annotated exons only. can I use this program for analysis

dg520 commented 4 years ago

Hi @ucbtsh6 ,

If you have the right FASTA and GTF pairs, you can apply IRFinder on non-model organisms. To find out what is a suitable customized GTF file, please read the manual here. On the same page, it also includes instructions on how to build the reference using your local (customized) FASTA and GTF files.

Best, Dadi