search

williamritchie / IRFinder

Detecting intron retention from RNA-Seq experiments
53 stars 25 forks source link

Spliced and Unspliced Reads Information #176

Open cinnamon6 opened 10 months ago

cinnamon6 commented 10 months ago

Hello! I was wondering if it is possible to retrieve the spliced and unspliced reads information (basically the numerator and denominator values using which the IRratio is calculated).

dg520 commented 10 months ago

@cinnamon6 Yes, those values are in the IRFinder output file. Please refer to this and see how the value in Column 20 (i.e. IR ratio) is calculated from the values in Columns 8, 9, 17 and 18.

cinnamon6 commented 8 months ago

Thank you for your reply! Apart from the intron depth, is it possible to get the counts information at the introns as well?