Open tanwei123456 opened 1 year ago
You have got the pan.fa and pan.gff
There is not 'pan_dir_result/R498_0-2M.gff.in_pv' because the pipeline can't find any gene (or CDS, exon) on the PAV which would be inserted into ref2.genome. If the pipeline can find genes on the PAV which would be inserted into ref.genome, it will generate gene_absolutly_in_pv.gff file. For example, in the example case, CN1_0-2M.gene_absolutly_in_pv.gff cantains the gene on the PAV which would be inserted in the ref1.genome. The ref1.update2.gff will be updated by adding itself and the CN1_0-2M.gene_absolutly_in_pv.gff information. The adding result is ref2.gff If there is no gene_absolutly_in_pv.gff, The ref2.update2.gff is the same as ref3.gff. It is no problem with the pangenome construction
Dear author, Hello,when I run
python3 ../Construct_pan_and_Call_sv.py genome_gff_dir_example genome_list -fqd fq_dir_example -o step1
, I am experiencing the following error:This is the resulting file.
R version:R-4.0.3 and Python version: python3.9.4.env I would appreciate it if you could answer my questions! tanwei 2023.03.15