Open TATABOX99 opened 5 days ago
作者您好，你的seurat计算部分使用的：sc.metabolism.Seurat <- function(obj, method = "VISION", imputation = F, ncores = 2, metabolism.type = "KEGG") {
countexp<-obj@assays$RNA@count
在seurat5.0中不可用了，此外考虑到批次效应，是否可以尝试改成：sc.metabolism.Seurat <- function(obj, method = "VISION", imputation = F, ncores = 2, metabolism.type = "KEGG") {
countexp<-obj@assays$RNA$data
作者您好，你的seurat计算部分使用的：sc.metabolism.Seurat <- function(obj, method = "VISION", imputation = F, ncores = 2, metabolism.type = "KEGG") {
countexp<-obj@assays$RNA@count
在seurat5.0中不可用了，此外考虑到批次效应，是否可以尝试改成：sc.metabolism.Seurat <- function(obj, method = "VISION", imputation = F, ncores = 2, metabolism.type = "KEGG") {
countexp<-obj@assays$RNA$data