wyang17 / SQuIRE

Software for Quantifying Interspersed Repeat Expression
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Error in Call step #61

Closed AbhijeetRPatil closed 3 years ago

AbhijeetRPatil commented 3 years ago

I am getting errors while running the call step in SquIRE. The counts were generated successfully without any errors. However, in the call step, I am getting errors. I have attached the errors below:

The following object is masked from ‘package:base:

apply

Warning message: multiple methods tables found for ‘pos’ [1] "/home/arpatil/dete/TEs_T1D_Normal_Squire_gene_TE_counttable.txt" [2] "/home/arpatil/dete/TEs_T1D_Normal_Squire_coldata.txt"
[3] "/home/arpatil/dete"
[4] "TEs_T1D_Normal_Squire"
[5] "4"
[6] "T1D"
[7] "Normal"
[8] "20"
Error in read.table(file = file, header = header, sep = sep, quote = quote, : duplicate 'row.names' are not allowed Calls: as.matrix -> read.delim -> read.table Execution halted Traceback (most recent call last): File "/home/arpatil/miniconda3/envs/squire/bin/squire", line 11, in load_entry_point('SQuIRE', 'console_scripts', 'squire')() File "/home/arpatil/Tools/SQuIRE/squire/cli.py", line 156, in main subargs.func(args = subargs) File "/home/arpatil/Tools/SQuIRE/squire/Call.py", line 337, in main create_rscript(counttable,coldata,outfolder,output_format,projectname,verbosity,str(pthreads),prefilter,condition1,condition2,label_no) File "/home/arpatil/Tools/SQuIRE/squire/Call.py", line 198, in create_rscript sp.check_call(["/bin/sh","-c",Rcommand]) File "/home/arpatil/miniconda3/envs/squire/lib/python2.7/subprocess.py", line 186, in check_call raise CalledProcessError(retcode, cmd) subprocess.CalledProcessError: Command '['/bin/sh', '-c', 'Rscript /home/arpatil/dete/TEs_T1D_Normal_Squire_R_script.tmpOGNGUJ /home/arpatil/dete/TEs_T1D_Normal_Squire_gene_TE_counttable.txt /home/arpatil/dete/TEs_T1D_Normal_Squire_coldata.txt /home/arpatil/dete TEs_T1D_Normal_Squire 4 T1D Normal 20']' returned non-zero exit status 1

I have the count's table generated. For ex, I got the following files: TEs_T1D_Normal_Squire_coldata.txt, TEs_T1D_Normal_Squire _counttable.txt, TEs_T1D_Normal_Squire _combo.txt files generated successfully. But, it is not giving the differentially expressed TEs output.

Could anyone please help me in addressing this error? Thanks!