wyguo / ThreeDRNAseq

A pipeline for differential expression and differential alternative splicing analysis
https://github.com/wyguo/ThreeDRNAseq
GNU General Public License v3.0
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PCA and data normalisation [Bug] #39

Open HendrikWS opened 2 years ago

HendrikWS commented 2 years ago

When attempting to perform a PCA an error occurs: "An error has occurred. Check your logs or contact the app author for clarification." The error only appears when a polygon is added to the clusters.

After removing batch effects using the RUVg method, the data normalisation fails.

Screenshot 2022-11-24 at 14 42 03

I also tested the Arabidopsis example data and the same error occurred.

I am not sure if this applicable, but I am currently trying to run 3D RNAseq on a mac with a arm64 processor. While previously everything worked fine using a mac with an intel processor.